Project description:Expression profiling of 3T3-F442A adipocytes treated with growth hormone (GH, 500 nM) or vehicle (DMEM + 1% BSA) control for 30 min., 4 hr., or 48 hr in three independent experiments. Chronic GH treatment induces metabolic changes consistent with insulin resistance in 3T3-F442A adipocytes. Keywords: time-course
Project description:Cultured Madin-Darby canine kidney (MDCK) cells treated with hepatocyte growth factor (HGF) for 0, 3 and 24 hours. Each time point in triplicate. Keywords: time-course
Project description:Growth hormone (GH) is important in the development and maintenance of bone; however the IGF-dependent and -independent molecular pathways involved remain to be established. We used microarray analysis to identify genes acutely regulated by GH, in the bones of four week old GH-deficient mice at 6 or 24 hours following a single injection of GH (4 mg/kg body wt) or PBS (n = 6/group). 6,160 genes were differentially expressed at P = 0.05 and 17% of these genes were identified at both time points. Several of the 201 genes that were up or down regulated at > 2 fold at 6 and/or 24 hours (P = 0.01) were expressed sequence tags, transcription factors, or signaling genes. In conclusion, GH caused acute changes in several novel genes, suggesting that many GH-induced signaling pathways and target genes remain to be discovered Keywords: GH injection induced gene expression, time course
Project description:Identification of hair cycle-associated genes from time-course gene expression profile data by using replicate variance. Proc. Natl. Acad. Sci. USA. (2004) Keywords: time-course
Project description:We used cDNA microarrays to describe the changes in gene expression in muscle during recovery growth induced by a fasting-refeeding sequence. Keywords: muscle, fasting-refeeding, time-course
Project description:In this study we performed transcriptional profiling of transurethral resections of hormone resistant prostate cancer and compared it with benign prostatic hyperplasia (BPH), untreated localized prostate cancer and hormone sensitive prostate cancer. Keywords: time course; disease state analysis