Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Lack of a role of NHE4 in renal ammonia metabolism

ABSTRACT: Kidneys from a male and female mouse were used for single nucleus isolation and transcriptome analyses using the 10X Genomics platform.

ORGANISM(S): Mus musculus

PROVIDER: GSE290102 | GEO | 2025/04/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Lack of a role of NHE4 in renal ammonia metabolism

Project description:Lack of a role of NHE4 in renal ammonia metabolism

| PRJNA1226205 | ENA

A comparative study of cellular diversity between the Xenopus pronephric and mouse metanephric nephron

Project description:To characterize the cellular makeup of the Xenopus kidney, we dissected, dissociated, then processed X. laevis pronephric kidneys through the 10X Genomics single-cell pipline.

2022-09-04 | GSE212518 | GEO

Single Cell RNAseq on Renal adenocarcinoma

Project description:We used droplet based Single cell RNA sequencing (10x genomics) on sorted cell from tumor and juxta tumor tissue to explore myeloid cell diversity and metabolism in tumor microenvironement

2020-11-16 | GSE159913 | GEO

Single cell RNA-Seq of sorted nephron progenitor cells from pooled mouse kidneys

Project description:Kidneys from multiple litters of Six2GFP+ E14.5 mouse embryos were pooled into three replicate tubes and dissociated in parallel. Six2GFP+ cells were isolated and processed for single cell sequencing using 10x Genomics technology. This resulted in a dataset of 7844 single cells representing the nephron progenitor population of the mouse kidney.

2019-05-30 | GSE130606 | GEO

Single cell RNASeq of whole mouse kidneys from Uromodulin/Tamm horsfall protein knock-out mice

Project description:To elucidate changes in cellular populations in the ischemic acute kidney injury, we performed single cell RNAseq on mouse kidneys following ischemic reperfusion injury and a sham surgery. Mice were subjected to bilateral clamping of the renal pedicule for 30 minutes. After 6-7 hours of reperfusion the kidneys were removed and processed by a MACs cell isolation protocol and dead cell removal (PMID:33448928) , sorted on a 10X Chromium platform version 3 and libraries generated. Sequencing was performed on an Illumina NovaSeq 6000.

2021-04-08 | GSE171639 | GEO

The neuronal architecture of autonomic dysreflexia

Project description:This submission is a dataset of single-nucleus transcriptomics in the spinal cords of mice. The single-nuclei transcriptomics data is harvested and profiled using 10x Genomics Chromium Single Cell Kit Version.

2025-02-21 | GSE256423 | GEO

Single-cell RNA-sequencing analysis for the molecular taxonomy of primate amygdala

Project description:We thus isolated amygdala from rhesus macaques and performed single-cell RNA-sequencing analysis. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3’ V2 Reagent Kits (10X Genomics, Pleasanton, USA). Briefly, cells nuclei were concentrated to 1000 nuclei/μL and approximately 15000 nuclei were loaded into each channel to generate single-cell Gel Bead-In-Emulsions (GEMs), which results into expected mRNA barcoding of 9000 single nucleus for each sample.

2020-11-19 | GSE145765 | GEO

Comparison of single-nucleus and single-cell transcriptomes in hepatocellular carcinoma tissue

Project description:In the present study, scRNA-seq(single-cell RNA sequencing) was compared with snRNA-seq(single-nucleus RNA sequencing) in tumor tissue obtained from patients with HCC, using the 10X Genomics Chromium platform.

2022-08-09 | GSE210679 | GEO

Single nucleus ATAC-seq datasets for "Temporally Specific Gene Expression and Chromatin Remodeling Programs Regulate a Conserved Pdyn Enhancer"

Project description:This dataset contains single nucleus Assay for Transposase-Accessible Chromatin (ATAC) sequencing results from rat nucleus accumbens tissue. Rats received repeated cocaine injections (20mg/kg, intraperitoneal injection), or saline injections as a control. Single-nucleus ATAC-seq was carried out with FACS-sorted nuclei using the 10X Genomics Chromium single cell sequencing platform using the Chromium Next GEM Single Cell ATAC Kit v2.

2023-08-07 | GSE233754 | GEO

Polymorphisms in Renal Ammonia Metabolism Genes Correlate With 24-Hour Urine pH.

Project description:IntroductionUrine pH is critical for net acid and solute excretion, but the genetic factors that contribute to its regulation are incompletely understood.MethodsWe tested the association of single nucleotide polymorphisms (SNPs) from 16 genes related to ammonia (NH3) metabolism (15 biological candidates selected a priori, 1 selected from a previous genome-wide association study analysis) to that of 24-hour urine pH in 2493 individuals of European descent across 2 different cohorts using linear regression, adjusting for age, sex, and body mass index.ResultsOf 2871 total SNPs in these genes, 13 SNPs in ATP6V0A4 (a4 subunit of hydrogen- adenosine triphosphatase), SLC9A3 (sodium/hydrogen exchanger, isoform 3), and RHCG (Rhesus C glycoprotein), and 12 SNPs from insulin-like growth factor binding protein 7 (IGFBP7) had a meta-analysis P value <0.01 in the joint analysis plus a consistent direction of effect and at a least suggestive association (P < 0.1) in both cohorts. The maximal effect size (in pH units) for each additional minor allele of the identified SNPs was -0.13 for IGFBP7, -0.08 for ATP6V0A4, 0.06 for RHCG, and -0.06 for SLC9A3; SNP rs34447434 in IGFBP7 had the lowest meta-analysis P value (P = 7.1 × 10-8). After adjusting for net alkali absorption, urine pH remained suggestively associated with multiple SNPs in IGFBP, 1 SNP in ATP6V0A4, and a new SNP in GLS (phosphate-dependent glutaminase).DiscussionOverall, these findings suggest that variants in common genes involved in ammonia metabolism may substantively contribute to basal urine pH regulation. These variations might influence the likelihood of developing disease conditions associated with altered urine pH, such as uric acid or calcium phosphate kidney stones.

| S-EPMC5733879 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data