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FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [smallRNA-seq]

ABSTRACT: Condensates that accumulate small RNA biogenesis factors (nuage) are common in germ cells and often associate with nuclei. In the C. elegans germline, P granules overlay large clusters of nuclear pores and this organization has been proposed to facilitate surveillance of nascent transcripts by Argonaute proteins enriched in P granules. We report that co-clustering of nuclear pores and P granules depends on FG repeat containing nucleoporins and FG repeats in the Vasa class helicase GLH-1. Mutants that prevent co-clustering are fertile under standard growth conditions and only mis-regulate a minority of genes, including replication-dependent histones. Our observations suggest that association with nuclear pores, while non-essential for genome surveillance, may serve to tune mRNA flow through P granules and other nuage condensates.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE291761 | GEO | 2025/03/16

REPOSITORIES: GEO

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FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [RNA-seq]

Project description:Condensates that accumulate small RNA biogenesis factors (nuage) are common in germ cells and often associate with nuclei. In the C. elegans germline, P granules overlay large clusters of nuclear pores and this organization has been proposed to facilitate surveillance of nascent transcripts by Argonaute proteins enriched in P granules. We report that co-clustering of nuclear pores and P granules depends on FG repeat containing nucleoporins and FG repeats in the Vasa class helicase GLH-1. Mutants that prevent co-clustering are fertile under standard growth conditions and only mis-regulate a minority of genes, including replication-dependent histones. Our observations suggest that association with nuclear pores, while non-essential for genome surveillance, may serve to tune mRNA flow through P granules and other nuage condensates.

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A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing

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FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [smallRNA-seq]

Project description:FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [smallRNA-seq]

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FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [RNA-seq]

Project description:FG repeats drive co-clustering of nuclear pores and P. granules in the C elegans germline [RNA-seq]

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Nucleoporin Nsp1 surveils the phase state of FG-Nups

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