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Transcriptome analyses of fetal testicular interstitial cells (W-EGFP) treated with Smoothened Agonist (SAG)

ABSTRACT: Fetal Leydig cells are essential for masculinization through androgen production during fetal development. Previous studies have indicated that Desert Hedgehog regulates differentiation from progenitor cells. Our previous research showed a significant upregulation of energy metabolism-related genes during fetal Leydig cell differentiation. However, the underlying regulatory mechanisms remain unresolved. Here, we reveal that Desert Hedgehog rapidly activates energy metabolisms in testicular interstitial cells (W-EGFP cells) containing fetal Leydig progenitor cells without activating gene expression.

ORGANISM(S): Mus musculus

PROVIDER: GSE292010 | GEO | 2025/03/24

REPOSITORIES: GEO

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CUT&RUN analyses of fetal Leydig cells and testicular interstitial cells

Project description:Ad4BP/SF-1 is a transcription factor regulating gene expression related to steroidogenesis and energy metabolism. Previous studies have indicated that Ad4BP/SF-1 regulates fetal Leydig cell differentiation from progenitor cells. However, the underlying regulatory mechanisms remain unclear. Here, we reveal that Ad4BP/SF-1 directly regulates energy metabolisms in differentiated fetal Leydig cells through transcriptional upregulation.

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Targeting resistance to Smoothened antagonists by FACT inhibition

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Purification and transcriptomic analysis of mouse fetal Leydig cells reveals candidate genes for disorders of sex development

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NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [ChIP-Seq]

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