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Impact of adcR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Zinc

ABSTRACT: Transcriptome comparison of the Streptococcus pneumoniae D39 ΔadcR to D39 wild-type grown in CDM plus 0.2mM Zn2+.

ORGANISM(S): Streptococcus pneumoniae D39

PROVIDER: GSE29234 | GEO | 2011/05/13

SECONDARY ACCESSION(S): PRJNA142941

REPOSITORIES: GEO

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Transcriptional response of Streptococcus pneumoniae D39 to Zinc

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in CDM Plus 0mM Zn2+ to grown in CDM plus 0.2 mM Zn2+.

2011-05-13 | GSE29235 | GEO

Impact of adcR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Ni2+

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type to D39 ΔadcR grown in CDM with 0.3 mM Ni2+

2015-11-20 | GSE73851 | GEO

adcR mutant vs wild type D39

Project description:Comparison of the Streptococcus pneumoniae D39 adcR mutant vs D39 wild type in CDM plus 0.2mM Zn One condition design comparison of two strains including a dye swap

2011-05-13 | E-GEOD-29234 | biostudies-arrayexpress

Impact of naiR delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔnaiR to D39 wild type grown in CDM

2017-02-24 | GSE94511 | GEO

Impact of copY delation on the transcriptome of Streptococcus pneumoniae D39

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 copY-stop mutant to D39 wild-type grown in CDM without added copper.

2011-07-10 | GSE30414 | GEO

D39 wild-type vs D39 ÎadcR In CDM with 0.3 mM Ni2+

Project description:By using the transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB and phtE) and the PsaR regulon (pcpA, prtA and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon as compared to Zn2+. Comparison of the Streptococcus pneumoniae D39 wild-type vs D39 ÎadcR in CDM Plus 0.3 mM Ni2+ Two condition design comparison of Wild-type strain vs mutant strain including a dye swap

2015-11-20 | E-GEOD-73851 | biostudies-arrayexpress

Impact of ccpA delation on the transcriptome of Streptococcus pneumoniae D39 in the presece of N-acetylglucosamine (NAG)

Project description:Transcriptome comparision of the Streptococcus pneumoniae D39 ccpA mutant to D39 wild-type grown in CDM + 0.5 % N-acetylglucosamine (NAG)

2017-01-12 | GSE89590 | GEO

Transcriptome response of the Streptococcus pneumoniae D39 wild-type to Ni2+

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grownin CDM with 0.5 mM Ni2+ to grown in CDM with 0 mM Ni2+

2015-11-20 | GSE73850 | GEO

Transcriptional response of Streptococcus pneumoniae to Zn2+-limitation and the repressor/activator function of AdcR

Project description:Zinc (Zn2+) is an important trace metal ion that has been shown to regulate the expression of several (virulence) genes in streptococci. Previously, we analyzed the genome-wide response of S. pneumoniae to Zn2+-stress. In this work, we have performed a transcriptomic analysis to identify genes that are differentially expressed under intracellular Zn2+-limitation. This revealed a number of genes that are highly upregulated in the absence of extracellular Zn2+, amongst which the genes belonging to the regulon of the Zn2+-responsive repressor AdcR, like adcBCA, encoding a Zn2+-dependent ABC-uptake system, adcAII, encoding a Zn2+-binding lipoprotein, and also virulence genes belonging to the Pht family (phtA, phtB, phtD and phtE). Using transcriptome analysis, lacZ-reporter studies, in vitro DNA binding experiments, and in silico operator predictions, we show that AdcR directly represses the promoters of adcRCBA, adcAII-phtD, phtA, phtB and phtE in the presence of Zn2+. AdcR can also function as an activator, since in the presence of Zn2+ it directly induces expression of adh that encodes a Zn2+-containing alcohol dehydrogenase. In conclusion, the genome-wide transcriptional response of S. pneumoniae to Zn2+-limitation was established, which is mainly mediated via direct regulation by the Zn2+-dependent regulator AdcR. Two condition design comparison of Wild-type strain including a dye swap Refer to individual Series. This SuperSeries is composed of the following subset Series: GSE29234: adcR mutant vs wild type D39 GSE29235: Low Zn vs high Zn in CDM

2011-05-13 | E-GEOD-29236 | biostudies-arrayexpress

Transcriptome response of the Streptococcus pneumoniae D39 wild-type to N-acetyleglucosamine (NAG)

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown at in CDM with 0.5% Glucose to grown in CDM with 0.5 % N-acetyleglucosamine (NAG)

2017-01-12 | GSE89589 | GEO

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