Transcriptomics

Dataset Information

0

MiR-21 involves in cervical squamous cell tumorigenesis and regulates CCL20


ABSTRACT: MicroRNA 21 (miR-21) has been implicated in various aspects of carcinogenesis. However, the function and molecular mechanism of miR-21 in cervical squamous carcinoma has not been studied. Using TaqMan quantitative real-time PCR and Northern blot, we confirmed that miR-21 is significantly overexpressed in human cervical squamous cancer tissues and cell lines. Remarkably, we showed that the level of miR-21 correlates with the nodal status and differentiation by ISH. Furthermore, we demonstrated that miR-21 regulates cervical squamous cells proliferation, apoptosis, and migration which are HPV16 positive. In order to identify the candidate target genes for miR-21, we used gene expression profiling. By luciferase reporter assay, we confirmed the CCL20 gene is one of its targets, which is relative to the HPV16 oncogenes E6 and E7. Our results suggest that miR-21 may be involved in the cervical squamous cell tumorigenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29242 | GEO | 2012/01/01

SECONDARY ACCESSION(S): PRJNA139955

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-01-01 | E-GEOD-29242 | biostudies-arrayexpress
| PRJNA139955 | ENA
2020-12-21 | GSE147009 | GEO
2021-02-10 | GSE166466 | GEO
2018-01-16 | E-MTAB-6361 | biostudies-arrayexpress
2023-05-25 | GSE228568 | GEO
| PRJNA550026 | ENA
2018-09-24 | PXD011058 | Pride
2012-06-27 | E-GEOD-30656 | biostudies-arrayexpress
2012-12-31 | E-GEOD-34575 | biostudies-arrayexpress