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Transcriptional profiling of Mycobacterium tuberculosis exposed to antibiotics and drug combinations in broth culture.

ABSTRACT: We profiled Mtb transcriptional response to a range of different antibiotic drug exposures in 7H9 broth culture by RNA-seq. Compound abbreviations: (EMB = ethambutol, BTZ = BTZ043, TYL = tyloxapol). We also hypothesized that an improved Mtb TRN could be inferred using a large, diverse compendium of RNA-seq data and therefore sought to supplement the existing repository of Mtb expression data available on SRA with additional conditions that could inform on Mtb's underlying regulatory programs. We went on to infer Mtb's TRN using a collection of inference methods in combination with this compendium, and employed it to build an interpretable machine learning model of Mtb fitness under stress.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE292636 | GEO | 2025/03/21

REPOSITORIES: GEO

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