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The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Pseudomonas protegens Pf-5 Pseudomonas fluorescens

PROVIDER: GSE29322 | GEO | 2011/05/16

SECONDARY ACCESSION(S): PRJNA140115

REPOSITORIES: GEO

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The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5.

Project description:One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by employing microarray and iTRAQ techniques, respectively. Analysis of this data revealed that genes encoding functions related to iron homeostasis, including pyoverdine and enantio-pyochelin biosynthesis, a number of TonB-dependent receptor systems, as well as some inner-membrane transporters, were significantly up-regulated in response to iron limitation. Transcription of a ribosomal protein L36-encoding gene was also highly up-regulated during iron limitation. Certain genes or proteins involved in biosynthesis of secondary metabolites such as 2,4-diacetylphloroglucinol (DAPG), orfamide A and pyrrolnitrin, as well as a chitinase, were over-expressed under iron-limited conditions. In contrast, we observed that expression of genes involved in hydrogen cyanide production and flagellar biosynthesis were down-regulated in an iron-depleted culture medium. Phenotypic tests revealed that Pf-5 had reduced swarming motility on semi-solid agar in response to iron limitation. Comparison of the transcriptomic data with the proteomic data suggested that iron acquisition is regulated at both the transcriptional and post-transcriptional levels.

| S-EPMC3377617 | biostudies-literature

The effect of Iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5: iron(III) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(III) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media

2011-05-16 | GSE29320 | GEO

The effect of iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5: iron(II) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(II) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media

2011-05-16 | GSE29319 | GEO

The effect of Iron limitation on the transcriptome and proteome of Pseudomonas fluorescens Pf-5: iron(III) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(III) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media Two-condition experiment, iron(III) chloride supplemented culture versus non-iron treated culture. 3 biological replicates including 3 technical replicates for one of the biological replicate and 2 technical replicates for another biological replicate. Swap-dye experiments were performed

2011-05-16 | E-GEOD-29320 | biostudies-arrayexpress

Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5

Project description:This SuperSeries is composed of the following subset Series: GSE29319: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride GSE29320: Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(III) chloride Refer to individual Series

2011-05-16 | E-GEOD-29322 | biostudies-arrayexpress

Tannic acid treatment effect on transcriptome of Pseudomonas fluorescens Pf-5

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-11-24 | GSE33909 | GEO

Iron-starvation effect on transcriptome of Pseudomonas fluorescens Pf-5: iron(II) chloride

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing iron(II) chloride supplemented grown culture against non-iron treated grown culture in M9 minimal media Two-condition experiment, iron(II) chloride supplemented culture versus non-iron treated culture. 4 biological replicates including 3 technical replicates for one of the biological replicates. Swap-dye experiments were performed

2011-05-16 | E-GEOD-29319 | biostudies-arrayexpress

The effect of zinc limitation on transcriptome expression in Pseudomonas fluorescens Pf-5

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing zinc-limited culture against zinc-amended culture in M9 minimal media Two-condition experiment, non-zinc treated culture versus zinc sulphate supplemented culture. 3 biological replicates including 3 technical replicates for one of the biological replicate and 2 technical replicates for another biological replicate. Swap-dye experiments were performed

2011-10-12 | E-GEOD-32921 | biostudies-arrayexpress

Tannic acid treatment effect on transcriptome of Pseudomonas fluorescens Pf-5

Project description:This SuperSeries is composed of the following subset Series: GSE33907: Tannic acid (20 µg/ mL) treatment effect on transcriptome of Pseudomonas fluorescens Pf-5 GSE33908: Tannic acid (160 µg/ mL) treatment effect on transcriptome of Pseudomonas fluorescens Pf-5 Refer to individual Series

2011-11-24 | E-GEOD-33909 | biostudies-arrayexpress

Tannic acid (160 µg/ mL) treatment effect on transcriptome of Pseudomonas fluorescens Pf-5

Project description:Transcriptomic profiling of Pseudomonas fluorescens Pf-5 comparing culture treated with 160 µg/mL tannic acid against non-treated culture grown in Mueller-Hinton media

2011-11-24 | GSE33908 | GEO

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