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CTCF regulates positioning of the human cystic fibrosis gene in association with a histone deacetylase

ABSTRACT: Treatment with the histone deacetylase inhibitor trichostatin a (TSA) changes the radial positioning of the CFTR gene in HeLa S3 cells. The gene relocates from the nuclear periphery to the nuclear interior. In Calu-3 cells the gene is located in the nuclear interior. To identifiy potential regulatory elements for the positioning of CFTR, the histone h3 and h4 acetylation patterns of untreated and TSA-treated HeLa S3 and untreated Calu-3 cells were determined by ChIP-chip. A CTCF site close to the CFTR promoter displayed consistent histone H3 hyperacetylation in TSA treated HeLa S3 cells and Calu-3 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29360 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA140191

REPOSITORIES: GEO

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CTCF regulates positioning of the human cystic fibrosis gene in association with a histone deacetylase

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