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Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation (gene expression)

ABSTRACT: SAGA is a highly conserved transcriptional co-activator complex involved in multiple steps of transcription with activities that function both pre and post initiation. Loss of individual subunits results in developmental defects, suggesting a role in development. In particular, SAGA contains a ubiquitin protease activity involved in the transition from transcription initiation to elongation. Loss of the Sgf11 SAGA subunit causes loss of deubiquitination activity, and leads to defects in axon targeting and embryonic lethality. To better understand the roles of SAGA functions in developmental gene expression we examined its composition, binding profile, and the effects of subunit loss on gene expression in two distinct cell types in late stage Drosophila embryos: muscle and neurons.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE29525 | GEO | 2011/08/22

SECONDARY ACCESSION(S): PRJNA154009

REPOSITORIES: GEO

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Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation (ChIP-seq)

Project description:SAGA is a highly conserved transcriptional co-activator complex involved in multiple steps of transcription with activities that function both pre and post initiation. Loss of individual subunits results in developmental defects, suggesting a role in development. To better understand the roles of SAGA functions in developmental gene expression and it's relationship with RNA polymerase II, we examined its composition, binding profile, and the effects of subunit loss on gene expression in two distinct cell types in late stage Drosophila embryos: muscle and neurons. Chromatin IP of FLAG-tagged SAGA subunit Ada2b, and RNA polymerase II (antibody 4H8), was performed in neuronal (elav+) or muscle (mef2+) cells isolated by FACS from late stage embryos, and compared to whole cell extracts (input). The control constists of an IP performed in neuronal cells from a non-tagged strain.

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Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation (ChIP-seq)

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DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

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DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis [ChIP-seq]

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