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Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough (tiling array)

ABSTRACT: High-resolution tiling analysis of the DvH transcriptome in lactate/sulfate media, both rich (LS) and defined (LS4D)

ORGANISM(S): Nitratidesulfovibrio vulgaris str. Hildenborough

PROVIDER: GSE29556 | GEO | 2011/05/27

SECONDARY ACCESSION(S): PRJNA154013

REPOSITORIES: GEO

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Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough (tiling array)

Project description:High-resolution tiling analysis of the DvH transcriptome in lactate/sulfate media, both rich (LS) and defined (LS4D) Two replicates of rich media, one replicate of minimal media, and one “genomic control” array (genomic_control.ftr) hybridized to genomic DNA

2011-05-27 | E-GEOD-29556 | biostudies-arrayexpress

Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough (RNA-Seq)

Project description:5' RNASeq of mRNA from DvH in defined lactate/sulfate media (LS4D)

2011-05-27 | GSE29559 | GEO

Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough (RNA-Seq)

Project description:5' RNASeq of mRNA from DvH in defined lactate/sulfate media (LS4D) One lane of sequence each for two different protocols applied to independently grown cells under similar conditions

2011-05-27 | E-GEOD-29559 | biostudies-arrayexpress

Using data from microarray experiments, we investigated the transcriptional changes in evolved and ancestor D. vulgaris strains.

2013-03-12 | GSE39675 | GEO

Effect of THPS on growth physiology and gene expression of Desulfovibrio vulgaris Hildenborough

Project description:The effect of tetrakis (hydroxymethyl) phosphonium sulfate (THPS) stress on Desulfovibrio vulgaris Hildenborough (DvH) gene expression was determined by comparing the gene expression profiles of DvH with THPS added at mid-log phase against DvH grown without THPS. Low concentration THPS had little influence over instantaneous growth rate constant and final cell density of the culture. Changes in gene expression caused by exposure to THPS were determined using full-genome DvH microarrays. In comparison to Glutaraldehyde and BAC microarray results, not lot of gene expressions were altered by THPS. Keywords: THPS Stress

2010-03-06 | GSE20657 | GEO

Effect of glutaraldehyde (Glut) on growth physiology and gene expression of Desulfovibrio vulgaris Hildenborough

Project description:The effect of glutaraldehyde stress on Desulfovibrio vulgaris Hildenborough (DvH) gene expression was determined by comparing the gene expression profiles of DvH with glutaraldehyde added at mid-log phase against DvH grown without glutaraldehdyde. Low concentration glutaraldehyde had little influence over instantaneous growth rate constant and final cell density of the culture. Changes in gene expression caused by exposure to glutaraldehyde were determined using full-genome DvH microarrays. A lot of gene expressions were altered by glutaraldehyde. Keywords: Glutaraldehyde Stress

2010-03-06 | GSE20656 | GEO

Evidence-based annotation of genes and transcripts in Desulfovibrio vulgaris Hildenborough

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-05-27 | GSE29560 | GEO

Using data from microarray experiments, we investigated the transcriptional changes in evolved and ancestor D. vulgaris strains.

Project description:Using data from microarray experiments, we investigated the transcriptional changes in evolved and ancestor D. vulgaris strains. gene expression changes in evolved salt-stressed DvH strain (ES, evolved in LS4D + 100 mM NaCl for 1200 generations), evolved control DvH strain (EC, evolved in LS4D for 1200 generations) and ancestor DvH strain grown in non-stress (LS4D), low salt stress (LS4D + 100 mM NaCl) or high salt stress (LS4D + 250 mM NaCl) conditions Comparison of gene expression in evolved salt-stressed strain (ES, 1200g) to ancestor (An) or evolved control (EC, 1200g) strains at mid-logarithmic phase under standard growth condition with defined medium LS4D, salt stress conditions LS4D+100 mM NaCl or LS4D+250 mM NaCl.

2013-03-12 | E-GEOD-39675 | biostudies-arrayexpress

Effect of sulfide on growth physiology and gene expression of Desulfovibrio vulgaris Hildenborough

Project description:The effect of sulfide stress on Desulfovibrio vulgaris Hildenborough (DvH) gene expression was determined by comparing the gene expression profiles of DvH under conditions in which sulfide was allowed to accumulate (high sulfide, average concentration 10 mM) against DvH cells grown under conditions in which sulfide was removed by continuous gassing (low sulfide, average concentration 1 mM). High sulfide significantly decreased the instantaneous growth rate constant and final cell density of the culture indicating a decreased bioenergetic fitness. Changes in gene expression caused by exposure to high sulfide were determined using full-genome DvH microarrays. The transcription of ribosomal protein-encoding genes was decreased, in agreement with the lower growth rate of DvH under high sulfide conditions. Interestingly, expression of the gene for DsrD, located downstream of the genes for dissimilatory sulfite reductase (DsrAB) was also strongly down-regulated. In contrast, the expression of many genes involved in iron accumulation, stress response and proteolysis, and chemotaxis were increased. This indicates that high sulfide represents a significant stress condition, in which the bioavailability of metals like iron may be lowered and in which proteins (e.g. metalloenzymes) may need to be refolded, or proteolytically degraded. Overall this leads to a reduced growth rate and less efficient biomass production with available resources.

2009-08-18 | GSE15073 | GEO

Evidence-based annotation of transcripts and proteins in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough.

Project description:We used high-resolution tiling microarrays and 5' RNA sequencing to identify transcripts in Desulfovibrio vulgaris Hildenborough, a model sulfate-reducing bacterium. We identified the first nucleotide position for 1,124 transcripts, including 54 proteins with leaderless transcripts and another 72 genes for which a major transcript initiates within the upstream protein-coding gene, which confounds measurements of the upstream gene's expression. Sequence analysis of these promoters showed that D. vulgaris prefers -10 and -35 boxes different from those preferred by Escherichia coli. A total of 549 transcripts ended at intrinsic (rho-independent) terminators, but most of the other transcripts seemed to have variable ends. We found low-level antisense expression of most genes, and the 5' ends of these transcripts mapped to promoter-like sequences. Because antisense expression was reduced for highly expressed genes, we suspect that elongation of nonspecific antisense transcripts is suppressed by transcription of the sense strand. Finally, we combined the transcript results with comparative analysis and proteomics data to make 505 revisions to the original annotation of 3,531 proteins: we removed 255 (7.5%) proteins, changed 123 (3.6%) start codons, and added 127 (3.7%) proteins that had been missed. Tiling data had higher coverage than shotgun proteomics and hence led to most of the corrections, but many errors probably remain. Our data are available at http://genomics.lbl.gov/supplemental/DvHtranscripts2011/.

| S-EPMC3187205 | biostudies-literature

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