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Expression Data from Toxoplasma gondii Infected Murine Bone Marrow-Derived Macrophages

ABSTRACT: We wanted to determine how type I ROP16 affect host gene expression We infected BMDM's from BALB/c with type I (RH) and type I (RH) ΔROP16

ORGANISM(S): Mus musculus

PROVIDER: GSE29582 | GEO | 2011/05/29

SECONDARY ACCESSION(S): PRJNA141415

REPOSITORIES: GEO

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Expression Data from Toxoplasma gondii Infected Murine Bone Marrow-Derived Macrophages

Project description:We wanted to determine how type I ROP16 affect host gene expression We infected BMDM's from BALB/c with type I (RH) and type I (RH) ?ROP16 BMDM's were plated, then infected with parasites for 18 hours. Then the RNA was harvested from the cells by Trizol.

2011-05-29 | E-GEOD-29582 | biostudies-arrayexpress

Expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived Macrophages

Project description:We wanted to determine how type II Toxoplasma GRA15 and type I ROP16 affect host gene expression. We infected bone marrow-derived macrophages (BMMs) from B6 mice with type II (Pru), type II +ROP16 I, type II Δgra15, or type II Δgra15 + ROP16I.

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Expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived Macrophages

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Project description:The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and regulates the expression of many host genes. However, many of genes lack known STAT3/6 transcription factor binding sites in their promoter regions. We wanted to understand what fraction of host genes that are modulated by ROP16 were dependent on the STAT3 and STAT6 signaling pathways. Bone marrow-derived macrophages (BMMs) from mLys-Mcre Stat3fl/fl mice were infected with type II (Pru A7), type II +ROP16 I, type II Δgra15, or type II Δgra15 + ROP16I and gene expression was analyzed 18 hrs after infection. This data set was generated side by side with infections performed in B6 BMDMs, the gene expression results for that experiment were previoiusly submitted under the GEO accession number GSE29404. In addition, gene expression of wild type B6 and Stat6-/- BMDMs were infected with the II+ROP16I strain.

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Project description:Toxoplasma strains are known to inhibit the expression of several interferon-gamma induced genes, and a type II strain was shown to dysregulate genome-wide responses to interferon-gamma in human fibroblasts (Kim et al., 2007, J Immunol.). In this study we aimed to determine the effect of infection with three clonal lineages of Toxoplasma, type I, II, and III strains on genome-wide interferon-gamma induced transcription in murine macrophages. We also assessed the effect of the two main Toxoplasma modulators of mouse macrophage transcription, ROP16 and GRA15 (Jensen et al., 2011, Cell Host Microbe). We used Affymetrix microarrays to analyze host cell transcription after Toxoplasma infection and interferon-gamma stimulation. RAW264.7 murine macrophages were left uninfected or infected with type I (RH), type I ?rop16 (RH ?rop16), type II (Pru), type II ?gra15 (Pru ?gra15), or type II (CEP) parasites at an MOI ~5 for 18 hours and subsequently stimulated with murine IFN-? for six hours. Plaque assays were done to assess parasite viability. Total RNA was isolated and hybridized to Affymetrix Mouse 430A 2.0 gene chips.

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