Project description:Human myeloid leukemia cell line, HL-60 cells, have a potential to differentiate into monocytes with treatment of 12-O-tetradecanoylphorbol-13-acetate (TPA). However, the underlying mechanism of differentiation by TPA is not fully elucidated yet. Here, we performed ChIP-seq profiling using RNA Pol II, HDAC2, AcH3 and H3K27me3, and analyzed the differential chromatin state changes during HL-60 differentiation by TPA. In this study, we focused on the atypical active genes, which showed enhanced H3 acetylation occupancies despite increased HDAC2 recruitment. We found that HDAC2 regulates the expression of these genes positively via histone deacetylase activity-independent manner. HDAC2 interacted and recruited Paired Box 5 (PAX5) to promoter of the target genes and regulated PAX5-mediated gene activation. Taken together, these data demonstrated the profiling of differentiation specific-chromatin status during the differentiation in HL-60 cells by TPA and suggested that HDAC2 activates certain genes through the interaction with PAX5 in enzyme activity independent pathway.
Project description:Transcriptional profiling of human leukemia HL-60 cells comparing ATRA treated HL-60 cells with ATRA plus ATO. Goal was to determine the effects of ATO on ATRA induced differentiation of HL-60 cells.
Project description:To understand the function of the small molecule Dip G, the gene expression profiles of HL-60 cells treated with Dip G were analyzed using the microarray.
Project description:Transcriptional profiling of human leukemia?HL-60 cells comparing ATRA treated HL-60 cells with ATRA plus ATO. Goal was to determine the effects of ATO on ATRA induced differentiation of HL-60 cells. Two-condition experiment, ATRA vs. ATRA plus ATO treated HL-60 cells.
Project description:RNA sequencing was performed to compare levels of intracellular transcripts in HL-60 WT and HL-60 HAX1 KO cells. Experiment was performed to check influence of HAX1 protein on cellular transcriptome (in HL-60 cell line)
Project description:Curcumin has antileukemic potential that is mediated by dfifferent pathways. Because we could not confirm that the Arylhydrocarbonreceptor, a ligand activated transcription factor, is involved in curcumins antileukemic effects, we performed microarray experiments to have an overall screening of the pathways affected by curcumin in HL-60 cells. We identified an involvement of the vitamin D receptor in the effects of curcumin on HL-60 cells by comparing our results with other experiments.