Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Crop Plant Integrates and Coordinates its Defenses to Cope with the Attack by Begomovirus and Whitefly

ABSTRACT: We analyzed the global defense responses in common tobacco against a begomovirus Tomato yellow leaf curl China virus, an invasive whitefly species Middle East-Asia Minor 1, and their co-infestation. The transcripts of defense related genes were both overlapping and divergent in response to virus and whitefly.

ORGANISM(S): Nicotiana tabacum

PROVIDER: GSE29812 | GEO | 2017/09/01

SECONDARY ACCESSION(S): PRJNA140927

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus (TYLCCNV)

Project description:We investigated the transcriptional response of invasive B. tabaci B biotype to tomato yellow leaf curl China virus (TYLCCNV) using Illumina sequencing technology. We found that 1,606 genes involved in 157 biochemical pathways were differentially expressed in the viruliferous whiteflies. Culture of B biotype whitefly was maintained on cotton plants. Three thousands of newly emerged adults of whitefly on cotton were released onto the leaves of healthy and viruliferous tobacco plants. They were allowed to feed for 24 h. After that, non-viruliferous and viruliferous whiteflies were transferred respectively to cotton plants in different cages and allowed to feed for 120 h. Then approximately 1,000 non-viruliferous and viruliferous female adults of whitefly were collected, respectively. The RNA was extracted and sequenced using Illunima Analyzer II.

2011-03-22 | E-GEOD-24374 | biostudies-arrayexpress

Genome-wide miRNA and piRNA profiles of the vector whitefly, Bemisia tabaci during feeding on Tomato yellow leaf curl virus-infected tomato

Project description:The whitefly, Bemisia tabaci MEAM1 is a devastating vector capable of transmitting hundreds of plant viruses, including Tomato yellow leaf curl virus (TYLCV), to important food and fiber crops. Here we performed genome-wide profiling of micro RNAs (miRNAs) and piwi-interacting RNAs (piRNAs) in whiteflies after feeding on TYLCV-infected tomato or uninfected tomato for 24, 48 and 72 h. Overall, 160 miRNAs were discovered, 68 of which were conserved and 92 were B. tabaci-specific miRNAs. Majority of the genes that were predicted to be targeted by miRNAs had gene ontologies related to metabolic processes. We identified two miRNAs that were differentially expressed in whiteflies when fed on TYLCV-infected tomato compared to whiteflies that fed on uninfected tomato. The identified piRNAs were expressed as clusters throughout the whitefly genome. A total of 53 piRNA clusters were expressed across all time points and treatments, while 5 piRNA clusters were exclusively expressed in whiteflies that fed on TYLCV-infected tomato, and 24 clusters were exclusively expressed in whiteflies that fed on uninfected tomato. Approximately 62% of all identified piRNAs were derived from non-coding sequences that included intergenic regions, introns, and UTRs with unknown functions. The remaining 38% of piRNAs were derived from coding sequences (CDS) and repeat elements. Transposable elements targeted by piRNA clusters included both class I retrotransposons such as Gypsy, Copia, and LINEs and class II DNA transposons such as MITE, hAT, and TcMar. Lastly, six protein coding genes were targeted in whiteflies that fed on TYLCV-infected tomato. Information on how TYLCV influences miRNA and piRNA expression in whiteflies provides a greater understanding of regulatory pathways involved in mediating whitefly-virus interactions, and will facilitate the identification of novel targets for RNAi control.

2019-02-28 | GSE111343 | GEO

Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus (TYLCCNV)

2011-03-22 | GSE24374 | GEO

The Immune Strategy and Stress Response of the Whitefly, Bemisia tabaci, to an Orally Delivered Bacterial Pathogen

Project description:The whitefly, Bemisia tabaci, a notorious agricultural pest, has complex relationships with diverse microbes. It recognizes and degrades pathogens, as other insects do, and also relies on endosymbionts for its survival. Both types of interaction have received great attention, because of their potential importance in developing novel whitefly control technologies. The recent developments in RNA-seq technology allows us to perform a comprehensive investigation of a whitefly’s defense responses after it has ingested the pathogen, Pseudomonas aeruginosa. Compared to uninfected whiteflies, 6 and 24 hour post-infected (hpi) whiteflies showed 1,348 and 1,888 differentially expressed genes, respectively. Functional analysis highlighted the involvement of mitogen associated protein kinase (MAPK) pathway in host-defense regulation. Three knottin-like antimicrobial peptide genes and several components of the humoral and cellular immune response were also activated, indicating that key immune elements recognized in other insect species are also important for the host response of B. tabaci. Our data also suggest that intestinal stem cell mediated epithelium renewal might be an important component of the whitefly’s defense against oral bacterial infection. In addition, we also show stress responses to be an essential component of the defense system. We identify for the first time the key immune-response elements utilized by B. tabaci against bacterial infection. This provides a framework for future research into the complex interactions between whiteflies and microbes.

2014-12-01 | GSE52837 | GEO

Silverleaf whitefly 2nd instar feeding on 7-week old Arabidopsis thaliana rosette leaves

Project description:Phloem-feeding pests cause extensive crop damage throughout the world yet little is understood about how plants perceive and defend themselves from these threats. The silverleaf whitefly (SLWF; Bemisia tabaci type B) is a good model for studying phloem-feeding insect-plant interactions as SLWF nymphs cause little wounding and have a long, continuous interaction with the plant. Using the Arabidopsis ATH1 GeneChip, the global responses to Silverleaf Whitefly 2nd instar feeding were examined. Keywords: stress response

2006-12-15 | GSE6516 | GEO

Understanding cross-communication between aboveground and below ground plant via transcriptome analysis of a sucking insect whitefly-infested pepper plants

Project description:Transcriptome analysis in response to infestation of whitefly in peppr leaf and root Microarry study using total RNA from whitefly infestation, BTH, whitefly + BTH, and control in both leaf and root of pepper

2014-01-01 | E-GEOD-52661 | biostudies-arrayexpress

Global analysis of the transcriptional difference between indigenous and invasive whiteflies reveals the possible mechanism of whitefly invasion

Project description:The whitefly Bemisa tabaci is a species complex of more than 31 cryptic species which include some of the most destructive invasive pests of many ornamental and glasshouse crops worldwide. Among them, Middle East-Asia Minor 1 (herein MEAM1) and Mediterranean (herein MED) have invaded many countries around the world and displaced the native whitefly species. However, the molecular differences between invasive and indigenous whiteflies remain largely unknown. The global transcriptional difference between the two invasive whitefly Bemisia tabaci species (MEAM1, MED) and one indigenous whitefly species (Asia II 3) were analyzed using the Illumina sequencing technology.

2013-11-24 | E-GEOD-41864 | biostudies-arrayexpress

Global analysis of the transcriptional difference between indigenous and invasive whiteflies reveals the possible mechanism of whitefly invasion

2013-11-24 | GSE41864 | GEO

Understanding cross-communication between aboveground and below ground plant via transcriptome analysis of a sucking insect whitefly-infested pepper plants

Project description:Transcriptome analysis in response to infestation of whitefly in peppr leaf and root

2014-01-01 | GSE52661 | GEO

Identification of Saliva Proteins of the Whitefly Bemisia tabaci

Project description:The whitefly Bemisia tabaci is a notorious pest of worldwide agriculture. It is believed to secrete saliva to counter plant defenses, but the underlying mechanism remains to be elucidated. Here, we characterize the gene/protein repertoires of B. tabaci salivary glands and secreted saliva by transcriptomic and LC–MS/MS analysis. A total of 698 salivary gland-higher expressed unigenes, as well as 172 saliva proteins are identified. Comparative analysis of the saliva composition in different arthropod species illustrates that proteins associated with binding, hydrolysis and oxidation-reduction are widely distributed in herbivorous saliva. There are 74 saliva proteins exclusively identified in B. tabaci, with 34 of them being B. tabaci-specific. In addition, eleven B. tabaci-specific saliva proteins plastically regulated in response to different diets, which might be associated with wide host range of this pest. Our results gain insight into whitefly–plant interactions, and provide a good resource for functional characterization of effectors

2020-01-15 | PXD016581 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data