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Gene expression data from cultured cells or from nude mice xenografts

ABSTRACT: In this study we performed gene transcription analysis of parental MACF7 cell and MCF7 engineered to overexpress oncoprotein RAF1. We demonstrate that overexpression of RAF1 leads to development of ivasive phenotype.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29884 | GEO | 2011/10/15

SECONDARY ACCESSION(S): PRJNA140739

REPOSITORIES: GEO

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Gene expression data from cultured cells or from nude mice xenografts

Project description:In this study we performed gene transcription analysis of parental MACF7 cell and MCF7 engineered to overexpress oncoprotein RAF1. We demonstrate that overexpression of RAF1 leads to development of ivasive phenotype. Total RNA was extracted from culturued cells or from nude mice xenografts.

2011-10-14 | E-GEOD-29884 | biostudies-arrayexpress

Gene expression data from MCF7 cells overexpressing RAF1

Project description:In this study we performed gene transcription analysis of parental MCF7 cells and MCF7 engineered to overexpress oncoprotein RAF1. We demonstrate that overexpression of RAF1 leads to development of ivasive phenotype.

2017-08-31 | GSE100360 | GEO

RNA-Seq of CTRL and PGR overexpressing MCF7 intraductal xenografts

Project description:Using a large variety of methods we demostrate that PGR can be a driver of tumor proliferation even in absence of extensive E2. In this specific experiment we overexpress PGR in MCF7 cells intraductally xenografted into the ductal tree of a mouse mammary gland, which provides post-menopausal levels of E2. Despite these conditions the cells thrive and with the PGR overexpression the growth rate is double the control cells. PGR overexpressing cells exhibit consistently more invasive and metastatic properties.

2022-04-05 | GSE192808 | GEO

Gene expression profiling of parental and TAMR MCF7 cells after knock-down of ER or FOXA1 or overexpresion of FOXA1

Project description:Gene expression profiles (RNA seq of parental MCF7 cells and tamoxifen resistant cells after ER or FOXA1 knock down and after overexpression of FOXA1 in parenatl cells).

2016-11-01 | GSE75329 | GEO

Human MCF7 cells engineered to overexpress MYF6 vs. Control cells containing empty vector constructs [Rec2]

Project description:Human MCF7 cells engineered to overexpress MYF6 vs. Control cells containing empty vector constructs [Rec2]

| PRJNA779370 | ENA

Human MCF7 cells engineered to overexpress MYF6 vs. Control cells containing empty vector constructs [Rec1]

Project description:Human MCF7 cells engineered to overexpress MYF6 vs. Control cells containing empty vector constructs [Rec1]

| PRJNA779085 | ENA

Identification of MAF controlled genes in breast cancer cells

Project description:To identify relevant genes transcriptionally controlled by MAF in breast cancer, we focused on genes whose expression changed accordingly to MAF transcriptiion factor overexpression in MCF7. We used comparative RNA hybridization (Affymetrix) to this end. Total RNA from parental and MAFshort or long isoform overexpressing MCF7 grown for 48 hours in regular media (see growth protocol) was isolated from in vitro cultured control and MAF expressing MCF7 parental cells. Total RNA was extracted using the TRIzolM-BM-. Plus RNA Purification Kit (Life Technologies).

2014-10-01 | E-GEOD-51231 | biostudies-arrayexpress

Homo sapiens

Project description:Gene expression data from MCF7 cells overexpressing RAF1

| PRJNA391451 | ENA

Genome-wide miRNA profile of MCF7 cells overexpressing wild-type and mutant miR-222

Project description:MCF7 cells were infected with retrovirus to overexpress wild-type and mutant miR-222

2015-02-05 | GSE64363 | GEO

Intravenously administered alphavirus vector VA7 eradicates orthotopic human glioma xenografts in nude mice.

Project description:BackgroundVA7 is a neurotropic alphavirus vector based on an attenuated strain of Semliki Forest virus. We have previously shown that VA7 exhibits oncolytic activity against human melanoma xenografts in immunodeficient mice. The purpose of this study was to determine if intravenously administered VA7 would be effective against human glioma.Methodology/principal findingsIn vitro, U87, U251, and A172 human glioma cells were infected and killed by VA7-EGFP. In vivo, antiglioma activity of VA7 was tested in Balb/c nude mice using U87 cells stably expressing firefly luciferase in subcutaneous and orthotopic tumor models. Intravenously administered VA7-EGFP completely eradicated 100% of small and 50% of large subcutaneous U87Fluc tumors. A single intravenous injection of either VA7-EGFP or VA7 expressing Renilla luciferase (VA7-Rluc) into mice bearing orthotopic U87Fluc tumors caused a complete quenching of intracranial firefly bioluminescence and long-term survival in total 16 of 17 animals. In tumor-bearing mice injected with VA7-Rluc, transient intracranial and peripheral Renilla bioluminescence was observed. Virus was well tolerated and no damage to heart, liver, spleen, or brain was observed upon pathological assessment at three and ninety days post injection, despite detectable virus titers in these organs during the earlier time point.ConclusionVA7 vector is apathogenic and can enter and destroy brain tumors in nude mice when administered systemically. This study warrants further elucidation of the mechanism of tumor destruction and attenuation of the VA7 virus.

| S-EPMC2799335 | biostudies-literature

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