Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Like-like hybridization to test 7K3 platform variability

ABSTRACT: This experiment compared separate pools of Drosophila which received identical topical treatments of 99% ethanol 6 hours after exposure. The purpose of this was to assess the degree of natural variability that could be expected to be observed between samples in the absence of distince treatments. This was to allow for greater confidence in interpreting the results of other experiments which compared insects that received different treatments. It was found that a differential expression log2ratio range of -1.41 to 1.41 encompassed over 99% of all genes in this experiment. Keywords: platform background variation evaluation

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE3036 | GEO | 2006/03/31

SECONDARY ACCESSION(S): PRJNA93047

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Evalutation of the effect of ethanol upon gene expression in adult Drosophila melanogaster

Project description:This experiment was intended to determine if topical exposure to ethanol led to significant changes in gene expression in adult Drosophila melanogaster. It was determined that topical exposure to ethanol did not lead to a strong differential expression of genes in Drosophila after 4 hours. The effect of topical treatment with 99% ethanol was compared to the effect of a water treatment which was used as a control and the differential expression between these two treatments was assessed four hours after treatment. Two biological replicates of each treatment were compared for a total of two microarray hybridizations

2010-06-11 | E-GEOD-3033 | biostudies-arrayexpress

Drosophila gene expression in response to the botanical insecticide Piper nigrum

Project description:This experiment was used to determine the effect of a botanical insecticide upon gene expression profiles in Drosophila melanogaster. Adult female Drosophila (oregon-R strain) were treated with an ethylacetate extract of Piper nigrum (Piperaceae) seeds formulated in 99% ethanol. Treatment was topical, using a Potter's tower to administer a total of 2 mL of a 0.9mg/mL concentration. Control treatment was identical except flies were treated with 99% ethanol as a solvent control. Gene expression was studied four hours post-treatment. The design is a direct comparison between total RNA of treated and untreated insects. Sample size is two and two reverse label hybridizations were done. Each sample is a pool of 240 insects which received the same treatment simultaneously.

2006-01-24 | E-GEOD-3032 | biostudies-arrayexpress

Drosophila gene expression in response to the botanical insecticide Piper nigrum

2006-01-24 | GSE3032 | GEO

Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Project description:A study was conducted to determine if different levels of vegetable and fish oils can alter antiviral responses of salmon macrophage-like cells (MLCs). Atlantic salmon were fed diets containing 7.38% (FO7) or 5.09% (FO5) fish oil. These diets were designed to be relatively low in EPA+DHA (i.e. FO7: 1.41% and FO5: 1%), but near the requirement level, resulting in no significant change in salmon growth. After a 16-week feeding trial, macrophages isolated from fish in these dietary groups were stimulated by a viral mimic (dsRNA: pIC) or PBS (control) for 24 h. A 44K microarray experiment identified the diet-responsive transcripts between dietary treatments and pIC-responsive transcripts within each group.

2017-10-31 | GSE93773 | GEO

Comparison of Genes in Imiquimod-treated and Control Tumors

Project description:The goal of the study is to examine changes in tumor gene expression after imiquimod treatment. RNA was extracted from spontaneous tumors in control mice (n=4) and in imiquimod-treated mice (n=4). Gene expression was compared between the control group and the treated group. In the imiquimod treatment group, the mice received topical treatment of 5% imiquimod cream (Aldara) on shaved skin at the site of spontaneous tumors for one treatment cycle (3 consecutive days). RNA was extracted from 4 spontaneous tumors from neu-tg mice treated with topical imiquimod for 1 cycle and 4 spontaneous tumors from control mice

2010-06-24 | E-GEOD-20032 | biostudies-arrayexpress

Evalutation of the effect of ethanol upon gene expression in adult Drosophila melanogaster

2006-03-31 | GSE3033 | GEO

Adult mouse lung digestion test

Project description:This experiment was performed to help optimize a digestion protocol for FACS of adult mouse lung cells. The sample JH2 was used to help generate lung epithelial cell signatures.

2023-08-24 | GSE200883 | GEO

Panicum virgatum cultivar:J348.B.1.41

Project description:Panicum virgatum J348.B.1.41 Resequencing

| PRJNA456074 | ENA

Comparison of Genes in Imiquimod-treated and Control Tumors

2010-01-26 | GSE20032 | GEO

Transcriptomics analysis of cortical astrocytes subjected to Rbpj-K deletion and stab-wound injury

Project description:We performed single cell RNA-sequencing to investigate the molecular properties of Rbpj-K deleted astrocytes from the mouse somatosensory cortex. In the presence of a stab-wound injury, these cells undertake a neurogenic program, which can be analysed in the present dataset. The experiment encompassed cells from Rbpj-K-/- as well as wild type animals, and collected from injured or healthy cortices 3 wpi.

2019-11-02 | GSE139842 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data