Transcriptomics

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Expression analysis of the singlet oxygen resistant 1 (sor1) mutant


ABSTRACT: In a screen for singlet oxygen resistant mutants, a mutant called sor1 was isolated with increased resistance to different oxidative and electrophilic stress conditions. This mutant was found to have a constitutive high expression of three known singlet oxygen response genes, a glutathione peroxidase GPX5 and two glutathione-S-transferase GSTS1 and GSTS2. The sor1 mutation was mapped to the gene of a 393-amino acid protein for which blast searches revealed only poor homology to other proteins but predicted a putative basic leucine zipper DNA binding domain indicating that it might function as transcription factor to activate gene expression during oxidative stress.Global expression analysis of sor1 compared to the untreated wild-type enabled to identify additional genes differentially regulated in the sor1 mutant including several other genes involved in oxidative stress response and detoxification of endogenous xenobiotics.

ORGANISM(S): Chlamydomonas reinhardtii

PROVIDER: GSE30645 | GEO | 2011/07/15

SECONDARY ACCESSION(S): PRJNA154675

REPOSITORIES: GEO

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