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Differential gene expression analysis between Miltefosine resistant and sensitive leishmania donovani

ABSTRACT: Gene expression analysis was carried out between experimental miltefosine resistant L.donovani (adapted to 30ug/ml miltefosine drug pressure) and its corresponding wild type miltefosine senstive parasite

ORGANISM(S): Leishmania Leishmania donovani

PROVIDER: GSE30685 | GEO | 2011/09/30

SECONDARY ACCESSION(S): PRJNA144437

REPOSITORIES: GEO

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Differential gene expression analysis between Miltefosine resistant and sensitive leishmania donovani

Project description:Gene expression analysis was carried out between experimental miltefosine resistant L.donovani (adapted to 30ug/ml miltefosine drug pressure) and its corresponding wild type miltefosine senstive parasite 3 biological replicates of lab generated miltefosine resistant Leishmania donovani (K417 M30) were compared with corresponding wild type sensitive parasite (K417WT)

2011-09-29 | E-GEOD-30685 | biostudies-arrayexpress

Differential Gene expression anslysis between artesunate sensitive and resistat Leishmania donovani

Project description:Gene expression analysis was carried out between experimental artesunate resistant Leishmania donovani (adapted to 50 µM artesunate drug pressure) and it's corresponding wild type artesunate sensitive parasite.

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Differential Gene expression anslysis between Paromomycin sensitive and resistat Leishmania donovani.

Project description:Gene expression analysis was carried out between experimental Paromomycin resistant Leishmania donovani (adapted to 100 µM paromomycin drug pressure) and it's corresponding wild type paromomycin sensitive parasite.

2016-11-01 | GSE74208 | GEO

Differential RNA expression profile in antimony resistant and antimony sensitive clinical isolates of Leishmania donovani

Project description:Infection with antimony resistant (SbR) but not with sentitive (SbS) Leishmania donovani (LD) gives rise to aggressive pathology and higher organ parasite load in infected patients, the cause of which is far from clear. The pentose phosphate pathway (PPP) is a key route of glucose metabolism in most organisms, providing NADPH for use as a cellular reductant and various carbohydrate intermediates used in cellular metabolism. Alteration in PPP is primary reason behind aggressive pathology and multi drug resistance in several types of carcinoma and other infectious disease model. A time kinetic study with equal inoculum of SbRLD and SbSLD promastigotes and host cells infected with equal number of SbR and SbSLD, resulted in remarkable higher growth rate in SbRLD which is also reflected in terms of their DNA content. Our result showed an activated PPP pathway in response to oxidative stress in SbRLD. Our result suggest that SbRLD have adapted to a higher nucleotide metaboism that provide them a selective survival advantage in presence of drug mediated oxidative stress and explains, in part, the cause of higher organ parasite load in SbRLD infected patients.

2020-02-04 | GSE144659 | GEO

Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [RNA expression profiling]

Project description:Drug resistance is a major public health challenge in Leishmaniasis chemotherapy, particularly in the case of emerging Leishmania/HIV-1 co-infections. Recently, we have delineated the mechanism of cell death induced by the HIV-1 protease inhibitor, Nelfinavir, in the Leishmania parasite. In order to investigate the underlying molecular mechanism involved in Nelfinavir resistance, in vitro Nelfinavir resistant amastigotes were developed by direct drug pressure in culture. RNA expression profiling analyses of closely related Leishmania species were used as a screening tool to compare Nelfinavir-resistant and -sensitive parasites in order to identify candidate genes involved in drug resistance, and several genes were found to be differentially expressed. Comparative gene hybridization (CGH) analyses of Nelfinavir-resistant and -sensitive Leishmania using whole-genome 60-mer oligonucleotide microarrays were also carried out. RNA expression profiles and the CGH of Nelfinavir resistant vs sensitive Leishmania amastigotes suggest that parasites regulate mRNA levels either by modulating gene copy numbers through chromosome aneuploidy, or gene deletion/duplication by homologous recombination. Interestingly, supernumerary chromosomes 6 and 11 in the resistant parasites lead to upregulation of the ABC class of transporters, which are involved in vesicular trafficking. Transporter assays using radiolabeled Nelfinavir suggest that the drug accumulates in greater amounts in the resistant parasites and in a time dependent manner. Furthermore, high-resolution electron microscopy showed an increased number of vacuoles in Nelfinavir-resistant parasites. Together these results suggest that Nelfinavir is rapidly and dramatically sequestered in these intracellular vesicles.

2013-06-27 | GSE42196 | GEO

Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [RNA expression profiling]

2013-06-27 | E-GEOD-42196 | biostudies-arrayexpress

Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [Comparative Genomic Hybridization (DNA) profiling]

2013-06-27 | GSE42195 | GEO

Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant [Comparative Genomic Hybridization (DNA) profiling]

2013-06-27 | E-GEOD-42195 | biostudies-arrayexpress

Leishmania donovani amastigotes: Nelfinavir (NFV)-sensitive (control) vs Nelfinavir-resistant

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-06-27 | GSE42197 | GEO

Overall gene expression comparison between antimony resistant and sensitive Leishmania donovani

Project description:The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani.

2016-01-01 | GSE62468 | GEO

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