Transcriptomics

Dataset Information

0

Comparison of gene expression profiles in naïve WT and Asc-null bone marrow derived dendritic cells


ABSTRACT: The adaptor protein ASC contributes to innate immunity through the assembly of caspase-1-activating inflammasome complexes. We demonstrate that ASC plays an inflammasome-independent cell-intrinsic role in adaptive immune cells. Asc-/- mice displayed defective antigen presentation by dendritic cells and lymphocyte migration due to impaired Rac-mediated actin polymerization. Genome-wide analysis showed that ASC, but not Nlrp3 or caspase-1, controls mRNA stability and expression of DOCK2, a guanine nucleotide exchange factor that mediates Rac-dependent signaling in immune cells. DOCK2-deficient dendritic cells showed similar defective antigen uptake as Asc-/- cells. Ectopic expression of DOCK2 in ASC-deficient cells restored Rac-mediated actin polymerization, antigen uptake and chemotaxis. Thus, ASC shapes adaptive immunity independently of inflammasomes by modulating DOCK2-dependent Rac activation and F-actin polymerization in dendritic cells and lymphocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE30769 | GEO | 2012/07/09

SECONDARY ACCESSION(S): PRJNA144331

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-07-08 | E-GEOD-30769 | biostudies-arrayexpress
2016-04-01 | E-GEOD-78755 | biostudies-arrayexpress
2009-01-14 | E-GEOD-14413 | biostudies-arrayexpress
2016-04-01 | GSE78755 | GEO
2011-08-20 | E-GEOD-25205 | biostudies-arrayexpress
2009-01-15 | GSE14413 | GEO
2014-12-31 | GSE56653 | GEO
2021-01-27 | PXD018638 | Pride
2023-11-25 | PXD041763 | Pride
2022-12-01 | PXD033002 | Pride