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Time course profiles in salt shock roots of Ceriops tagal

ABSTRACT: Transcriptional differentiation in response to environmental stresses plays critical roles in adaptation. Mangroves are dominant in intertidal zones and form dense forest at the sea-land interface, but little is known about the impact of transcriptional phenotype on their adaptation to the saline environments. To address this issue, we prepared customized chips containing cDNA from the root cDNA library of a mangrove species, Ceriops tagal, and then monitored the time-course transcript profiles in the roots by conducting a series of microarray experiments. Five durations of salt shock with 500 mM NaCl, namely 2, 5, 10, 24 and 192 hours, were included. A total of 88 unigenes that were recognized to be up- or down-regulated by salt shock and both gene categories in relation to stresses-induced responses and transcription factor activity were over-predominant. The stresses-responsive genes were significantly overrepresented, some of which were regulated in differential manners from their homologues genes in Arabidopsis. Specific transcriptional regulations were employed by C. tagal to cope with salt shock, which could benefit the salt-tolerant lifestyle of this mangrove species and possibly contribute to establishment of adaptation to the saline environments.

ORGANISM(S): Ceriops tagal

PROVIDER: GSE30909 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA146319

REPOSITORIES: GEO

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Project description:Sinonovacula constricta is an economically important bivalve species in China, Korea and Japan. It widely resides in estuarine and coastal areas where salinity fluctuates rapidly. However, little is known about its adaptation mechanisms to acute salt stresses. To address this, we establish nine cDNA libraries (triplicate each treatment) from juvenile S. constricta, which were subjected to low salinity (5 psu), optimal salinity (15 psu, the control) and high salinity (25 psu) for 6 h, respectively. Illumina sequencing generated 478,587,310 clean reads totally, which were assembled into 427,057 transcripts of 246,672 unigenes. Compared with the control, 1259 and 2163 differentially expressed genes (DEGs) were identified under acute low and high salt stresses, respectively. GO and KEGG enrichment analyses of DEGs suggested that several key metabolic modulations were mainly responsible for the acute salt stresses. Based on the significantly highlighted KEGG pathways, some key DEGs were identified and discussed in details, including cysteine sulfinic acid decarboxylase, alanine transaminase, glutamine synthetase, glutamate dehydrogenase, nitric-oxide synthase, arginase, betaine-homocysteine S-methyltransferase, serine racemase, L-serine ammonia-lyase, phosphoserine phosphatase, glutamate 5-kinase, glutamate-5-semialdehyde dehydrogenase, ornithine-oxo-acid transaminase, ornithine decarboxylase, stearoyl-CoA desaturase, carnitine O-palmitoyltransferase 1, serine/threonine-protein kinase, NF-kappa-B, NF-kappa-B inhibitor alpha, phosphoenolpyruvate carboxykinase and carbohydrate response element binding protein. Furthermore, some potential osmolytes were speculated. In summary, this study would not only provide insights into the adaptation mechanisms to acute salt stresses in juvenile S. constricta, but also facilitate to better understand the physical and biochemical performances of this bivalve species involved in acute salt stresses. Moreover, the transcriptome data obtained here greatly enriched the genetic information of S. constricta, which would be valuable for promoting its molecular biology researches.

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Time course profiles in salt shock roots of Ceriops tagal

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