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Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

ABSTRACT: Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Porcine circovirus type 2 (PCV2) has been identified as the causal agent of postweaning multisystemic wasting syndrome, an economically important multifactorial disease of the swine industry worldwide. We used microarrays to study the transcriptome of PAMs infection with PCV2.

ORGANISM(S): Sus scrofa

PROVIDER: GSE30918 | GEO | 2011/07/27

SECONDARY ACCESSION(S): PRJNA146315

REPOSITORIES: GEO

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Genome-wide transcriptional response of primary alveolar macrophages (PAMs) following infection with porcine circovirus type 2

Project description:Porcine alveolar macrophages (PAMs) play impoartant role in innate immunity. Porcine circovirus type 2 (PCV2) has been identified as the causal agent of postweaning multisystemic wasting syndrome, an economically important multifactorial disease of the swine industry worldwide. We used microarrays to study the transcriptome of PAMs infection with PCV2. PAMs were collected by bronchoalveolar lavage from health piglets (free of PCV2, PRRSV, PRV, CSFV, PPV), and PAMs were cultured for 48 hours and inoculated with 5 moi of PCV2.

2011-07-26 | E-GEOD-30918 | biostudies-arrayexpress

Expression data from mediastinal lymph nodes of piglets experimentally infected with porcine circovirus type 2 (PCV2)

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Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2)

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Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2)

Project description:This study aimed to characterize differences in gene expression in piglets inoculated with porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS). Comparisons between control and PCV2-inoculated pigs were done at five different time points: 0, 7, 14, 21 and 29 days post-inoculation. Keywords: time course Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n=4) and inoculated with 105.2 TCID50 of the Burgos PCV2 isolate (n=4). Pigs were bled at 0, 7, 14, 21, and 29 days post-inoculation.

2009-12-31 | E-GEOD-14790 | biostudies-arrayexpress

Transcription profiling of pig mediastinal lymph nodes from piglets experimentally infected with porcine circovirus type 2 (PCV2)

Project description:This study aimed to characterize differences in gene expression in piglets inoculated with porcine circovirus type 2 (PCV2), the essential causative agent of postweaning multisystemic wasting syndrome (PMWS). Comparisons between control and PCV2-inoculated pigs were done at five different time points: 1, 2, 5, 8, and 29 days post-inoculation. Experiment Overall Design: Seven-day-old caesarean-derived, colostrum-deprived piglets were distributed into two groups: control (n=8) and inoculated with 105.2 TCID50 of the Burgos PCV2 isolate (n=16). One control and 3 inoculated pigs were necropsied on days 1, 2, 5, and 8 post-infection (p.i.), the remaining pigs (4 of each group) were necropsied on day 29 p.i.

2009-11-15 | E-GEOD-14758 | biostudies-arrayexpress

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Project description:Postweaning multisystemic wasting syndrome in pigs has devastated the swine industry since the 1990s. Porcine circovirus type 2 (PCV2) is the primary cause of this disease. MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in regulating gene expression, especially at the post-transcription level. The expression profiles of miRNAs have been reported in porcine reproductive and respiratory syndrome, porcine parvovirus, and other pig diseases; however, the miRNA expression profiles in pigs infected with PCV2 have not been reported so far. The Laiwu pig (a Chinese indigenous pig breed) has different meat quality, adipogenesis, and disease-resistance from western commercial pig breeds. In this study, four small RNA libraries were constructed from the lung tissue of uninfected and infected Laiwu and Yorkshire/Landrace crossbred (YL) pigs. High-throughput sequencing and bioinformatics were used to determine the abundance and differential expression of miRNAs in the four libraries

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2012-06-30 | E-GEOD-22782 | biostudies-arrayexpress

PCV2 and CSFV coinfection LC-MSMS

Project description:Based on the coinfection model of porcine circovirus type 2 (PCV2) and classical swine fever virus (CSFV), iTRAQ with LC-MS/MS was applied to map the cellular proteome profiles and explore cellular responses of cells coinfected with PCV2 and CSFV.

2017-04-06 | PXD005125 | Pride

Expression data from mediastinal lymph nodes and blood samples of piglets experimentally infected with PCV2

Project description:This SuperSeries is composed of the following subset Series: GSE14758: Expression data from mediastinal lymph nodes of piglets experimentally infected with porcine circovirus type 2 (PCV2) GSE14790: Expression data from blood samples of piglets experimentally infected with porcine circovirus type 2 (PCV2) Refer to individual Series

2009-12-30 | E-GEOD-14791 | biostudies-arrayexpress

Transcriptome sequencing of PCV2 ORF5 overexpressed PK15 cells

Project description:This study investigated the immunological function of PCV2 ORF5 by ectopic expression of PCV2 ORF5 in PK15 cell line. Identifying the functional role of each PCV2 ORF associated with host cell modulation may provide better knowledge about the pathogenesis of postweaning multisystemic wasting syndrome (PMWS). PCV2 ORF5 is recently identified and the functional role of ORF5 during the pathogenesis after PCV2 infection is largely unknown.

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