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C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

ABSTRACT: Transcriptional profiling of C. elegans worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the nematode Pristionchus pacificus (see NCBI GEO series GSE31861) , to study evolution of developmental pathways regulating dauer development.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE30977 | GEO | 2011/07/28

SECONDARY ACCESSION(S): PRJNA155497

REPOSITORIES: GEO

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C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

Project description:Transcriptional profiling of C. elegans worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the nematode Pristionchus pacificus (separate data-sets on a custom microarray platform designed by us and manufactured by Agilent) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Total samples from both experiments 1 and 2 = 8.

2011-07-27 | E-GEOD-30977 | biostudies-arrayexpress

P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

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