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Expression data from yeast (wild type, rlm1 and swi3 mutants) exposed to Congo Red

ABSTRACT: We did transcription profiling on the effect of rlm1 (MAPK Slt2 transcription factor) deletion and swi3 (component of SWI/SNF complex involved in chromatin remodeling) deletion in genes involved in cell wall stress (Congo Red) response.

ORGANISM(S): Schizosaccharomyces pombe Saccharomyces cerevisiae

PROVIDER: GSE31176 | GEO | 2012/05/25

SECONDARY ACCESSION(S): PRJNA144769

REPOSITORIES: GEO

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Expression data from yeast (wild type, rlm1 and swi3 mutants) exposed to Congo Red

Project description:We did transcription profiling on the effect of rlm1 (MAPK Slt2 transcription factor) deletion and swi3 (component of SWI/SNF complex involved in chromatin remodeling) deletion in genes involved in cell wall stress (Congo Red) response. Three biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. absence of the drug 2. presence of 30 µg/ml of Congo Red

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Project description:S. cerevisiae was grown on YEPD. For Congo Red experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 μg/ml. At this time cells were collected t=0h. Keywords: repeat sample

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