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Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Saccharomyces cerevisiae Sigma1278b Saccharomyces cerevisiae

PROVIDER: GSE31393 | GEO | 2011/09/05

SECONDARY ACCESSION(S): PRJNA145877

REPOSITORIES: GEO

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Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies.

Project description:Eukaryotic cells contain nontranslating messenger RNA concentrated in P-bodies, which are sites where the mRNA can be decapped and degraded. We present evidence that mRNA molecules within yeast P-bodies can also return to translation. First, inhibiting delivery of new mRNAs to P-bodies leads to their disassembly independent of mRNA decay. Second, P-bodies decline in a translation initiation-dependent manner during stress recovery. Third, reporter mRNAs concentrate in P-bodies when translation initiation is blocked and resume translation and exit P-bodies when translation is restored. Fourth, stationary phase yeast have large P-bodies containing mRNAs that reenter translation when growth resumes. The reciprocal movement of mRNAs between polysomes and P-bodies is likely to be important in the control of mRNA translation and degradation. Moreover, the presence of related proteins in P-bodies and maternal mRNA storage granules suggests this mechanism is widely adapted for mRNA storage.

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Reconsidering movement of eukaryotic mRNAs between polysomes and P bodies.

Project description:Cell survival in changing environments requires appropriate regulation of gene expression, including posttranscriptional regulatory mechanisms. From reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P bodies and can return to active translation. We present evidence contradicting the notion that reversible storage of nontranslating mRNAs is a widespread and general phenomenon. First, genome-wide measurements of mRNA abundance, translation, and ribosome occupancy after glucose withdrawal show that most mRNAs are depleted from the cell coincident with their depletion from polysomes. Second, only a limited subpopulation of translationally repressed transcripts, comprising fewer than 400 genes, can be reactivated for translation upon glucose readdition in the absence of new transcription. This highly selective posttranscriptional regulation could be a mechanism for cells to minimize the energetic costs of reversing gene-regulatory decisions in rapidly changing environments by transiently preserving a pool of transcripts whose translation is rate-limiting for growth.

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Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

Project description:This SuperSeries is composed of the following subset Series: GSE31220: Polysome-associated mRNA levels upon glucose repletion GSE31392: Timecourse of total and polysome-associated mRNA levels post glucose deprivation Refer to individual Series

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Project description:Reconsidering Movement of Eukaryotic mRNAs Between Polysomes and P-bodies

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Translationally inhibited mRNAs control cell movement as untranslated sequences

Project description:This SuperSeries is composed of the SubSeries listed below.

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