Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide binding of STAT3 in peritoneal macrophages

ABSTRACT: To try to identify the mechanism of STAT3âs indirect action we have used a genomic approach to map the binding sites of STAT3 within the genome and also used RNA-seq technology to map the changes in RNA expression and transcript isoforms in response to IL-10.

ORGANISM(S): Mus musculus

PROVIDER: GSE31529 | GEO | 2012/02/13

SECONDARY ACCESSION(S): PRJNA154981

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Genome-wide binding of STAT3 in peritoneal macrophages

Project description:To try to identify the mechanism of STAT3âs indirect action we have used a genomic approach to map the binding sites of STAT3 within the genome and also used RNA-seq technology to map the changes in RNA expression and transcript isoforms in response to IL-10. Examination of STAT3 binding by ChIP-seq in Unstimulated and IL-10 treated peritoneal exudate macrophages purified from mice. We sequenced anti-STAT3 ChIP-seq as well as corresponding control (Input) libraries

2012-02-13 | E-GEOD-31529 | biostudies-arrayexpress

Transcriptome changes in IL-10 treated peritoneal macrophages

2012-02-13 | GSE31530 | GEO

Transcriptome changes in IL-10 treated peritoneal macrophages

Project description:To try to identify the mechanism of STAT3s indirect action we have used a genomic approach to map the binding sites of STAT3 within the genome and also used RNA-seq technology to map the changes in RNA expression and transcript isoform abundance in response to IL-10. Examination of transcriptome changes in peritoneal macrophages when treated with IL-10 for 4 hours. RNA was extracted and sequenced.

2012-02-13 | E-GEOD-31530 | biostudies-arrayexpress

Genome-wide analysis of STAT3-binding and gene expression changes in peritoneal macrophages upon interleukin-10 stimulation

Project description:This SuperSeries is composed of the following subset Series: GSE31529: Genome-wide binding of STAT3 in peritoneal macrophages GSE31530: Transcriptome changes in IL-10 treated peritoneal macrophages Refer to individual Series

2012-02-13 | E-GEOD-31531 | biostudies-arrayexpress

Genome Wide Binding profile and transcriptome of STAT3 and STAT3 Mutant Y640F in STAT3 Wildtype and STAT3 Mutant Y640F hematopoietic progenitor cell lines

Project description:In many human cancers the transcription factor STAT3 is constitutively active and contributes to oncogenesis, tumour growth and progression. Recently, gain-of-function mutations of STAT3 have been identified in patients suffering from various haematopoietic malignancies and are postulated to enhance the transcriptional activity of STAT3. We investigated the gene expression and binding profile of the most common STAT3 mutant Y640F compared to wild-type STAT3, describing it's mechanism of action and pinpointing to novel therapeutic intervention sites.

2023-01-25 | GSE211111 | GEO

Expression profiling of STAT3-regulated genes in U937-derived macrophages

Project description:We used RNA-Seq assay to characterize STAT3-regulated gene expression patterns in U937-derived macrophages. We wanted to determine how STAT3 activation involves in function, differentiation, cytokine secretion of macrophages.

2023-10-26 | GSE231829 | GEO

Genome-wide analysis of STAT3 mediated transcription reveals immune disease associated SNPs in STAT3 binding sites

Project description:The development of therapeutic strategies to combat immune-associated diseases requires the molecular mechanisms of human Th17 cell differentiation to be fully identified and understood. To investigate transcriptional control of Th17 cell differentiation we used primary human CD4+ T cells in siRNA-mediated gene silencing and chromatin-immunoprecipitation followed by massive parallel sequencing (ChIP-seq) to identify both the early direct and indirect targets of STAT3. The integrated data set presented in this study confirms that STAT3 is critical for transcriptional regulation of early human Th17 cell differentiation. Importantly, we found that a large number of SNPs from loci associated with immune mediated disorders were located at sites where STAT3 binds to induce Th17 cell specification. Introduction of such SNPs alters STAT3 binding in DNA Affinity Precipitation Assays. Overall, our study provides important new insights for modulating Th17-mediated pathogenic immune responses in humans.

2017-05-15 | GSE96912 | GEO

Transcriptional Roles for STAT3 in Leptin Action

Project description:Transcriptional Roles for STAT3 in Leptin Action

| PRJNA510419 | ENA

Genome wide map of Stat3 binding sites in C6 rat glioma cells [NimbleGen]

Project description:We report a map of Stat3 binding sites in C6 rat glioma cells. The data was obtained using whole genome technology using NimbleGen microarrays. Examination of Stat3 binding sites in C6 rat glioma cell line

2015-05-07 | E-GEOD-47020 | biostudies-arrayexpress

RNA-seq analysis of IL-10 treatment of wildtype and Ptpn1-/- knockout peritoneal macrophages

Project description:We report here the deep sequencing of the mRNA from peritoneal exudate cells (macrophages) purified from wildtype or Ptpn1 (PTP1B) knockout mice, either treated or untreated with IL-10. In periotenal macrophages IL-10 activates the transcription factor STAT3 to execute and anti-inflammatory gene expression programme. The tyrosine phosphatase PTPN1 targets STAT3 for dephosphorylation and leads to the deactivation of STAT3. In this study we examined the role of PTP1B in controlling the normal homeostatic level phosphorylation of STAT3 by comparing the IL-10/STAT3-mediated anti-inflammatory gene expression programme. We find that loss of PTP1B leads to an up-regulation of the activity of STAT3, both at the level of phosphorylation and also in enhanced expression of anti-inflammatory gene products. RNA-seq of wildtype and Ptpn1 (PTP1B) knockout mouse peritoneal macrophages, treated or untreated with IL-10

2014-05-12 | E-GEOD-49449 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data