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ChIP-chip from Drosophila egg chambers using MCM2-7 antibody

ABSTRACT: Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE31889 | GEO | 2011/09/07

SECONDARY ACCESSION(S): PRJNA155077

REPOSITORIES: GEO

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ChIP-chip from Drosophila egg chambers using MCM2-7 antibody

Project description:Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation Comparison of MCM2-7 ChIP sample compared to input DNA for Drosophila egg chambers

2011-09-06 | E-GEOD-31889 | biostudies-arrayexpress

ChIP-chip from Drosophila egg chambers using ORC2 antibody

2011-05-27 | GSE29517 | GEO

ChIP-chip from Drosophila egg chambers using ORC2 antibody

2011-09-07 | GSE31890 | GEO

ChIP-chip from Drosophila egg chambers using ORC2 antibody

Project description:Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC localizes to the six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified loci Comparison of ORC2 ChIP sample compared to input DNA for two different Drosophila strains and two developmental stage samples

2011-05-27 | E-GEOD-29517 | biostudies-arrayexpress

ChIP-chip from Drosophila egg chambers using ORC2 antibody

Project description:Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC does not localize to DAFC-34B after stage 10, despite a second round of replication initiation Comparison of ORC2 ChIP sample compared to input DNA for Drosophila egg chambers

2011-09-06 | E-GEOD-31890 | biostudies-arrayexpress

ChIP-chip from dissected Drosophila egg chambers using antibody recognizing RNAPII

Project description:Chromatin immunoprecipitation of RNAPII followed by hybridization to genome-wide tiling microarrays demonstrated that RNAPII localizes to all six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified genomic loci

2011-05-27 | GSE29518 | GEO

ChIP-chip from dissected Drosophila egg chambers using antibody recognizing RNAPII

2011-05-27 | E-GEOD-29518 | biostudies-arrayexpress

ChIP-chip from Drosophila egg chambers using antibody recognizing tetra-acetylated histone H4

Project description:Chromatin immunoprecipitation of tetra-acetylated histone H4 followed by hybridization to genome-wide tiling microarrays demonstrated that tetra-acetylated H4 is enriched at the two chorion Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified loci

2011-05-27 | GSE29520 | GEO

ChIP-chip from Drosophila egg chambers using antibody recognizing tetra-acetylated histone H4

2011-05-27 | E-GEOD-29520 | biostudies-arrayexpress

Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2

Project description:Minichromosome maintenance (MCM) proteins facilitate replication by licensing origins and unwinding the DNA double strand. Interestingly, the number of MCM hexamers greatly exceeds the number of firing origins suggesting additional roles of MCMs. Here we show a hitherto unanticipated function of MCM2 in cilia formation in human cells and zebrafish that is uncoupled from replication. Zebrafish depleted of MCM2 develop ciliopathy-phenotypes including microcephaly and aberrant heart looping due to malformed cilia. In non-cycling human fibroblasts, loss of MCM2 promotes transcription of a subset of genes, which cause cilia shortening and centriole overduplication. Chromatin immunoprecipitation experiments show that MCM2 binds to transcription start sites of cilia inhibiting genes. We propose that such binding may block RNA polymerase II-mediated transcription. Depletion of a second MCM (MCM7), which functions in complex with MCM2 during its canonical functions, reveals an overlapping cilia-deficiency phenotype likely unconnected to replication, although MCM7 appears to regulate a distinct subset of genes and pathways. Our data suggests that MCM2 and 7 exert a role in ciliogenesis in post-mitotic tissues.

2018-10-20 | GSE120766 | GEO

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