The differences of the transcriptional profile between wide-type maize and transgenic ZmPIS maize (drought tolerance) by the assay of digital gene expression profile data
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ABSTRACT: We report the application of single-molecule-based sequencing technology for high-throughput profiling of transcriptome in mazie plants. The ZmPIS gene coding PtdIns synthase from maize with a maize ubiquitin promoter was transferred into maize. The transgenic ZmPIS maize showed enhanced drought tolerance compared to non-transgenic maize. The differentially expressed genes between wide-type maize and transgenic ZmPIS maize were detected by the assay of digital gene expression profile and real time RT-PCR datas. The results displayed that the overexpression of ZmPIS resulted in the expression levels changes of a large number of genes including genes involved in the phosphatidylinositol (PI) metabolic pathway, photosynthesis metabolism, carbohydrate metabolism, aminoacid metabolism and genes coding transcription factors.
ORGANISM(S): Zea mays
PROVIDER: GSE31944 | GEO | 2013/09/01
SECONDARY ACCESSION(S): PRJNA145007
REPOSITORIES: GEO
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