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The response of PBMCs and primary airway epithelial cells to Influenza and RSV virus

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE32140 | GEO | 2012/03/12

SECONDARY ACCESSION(S): PRJNA147663

REPOSITORIES: GEO

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The response of PBMCs and primary airway epithelial cells to Influenza and RSV virus

Project description:This SuperSeries is composed of the following subset Series: GSE32137: The response of murine primary airway epithelial cells to Influenza infection and the importance of Interferon type I signaling in this response [mAEC]. GSE32138: The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Agilent]. GSE32139: The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina] GSE34205: Transcriptional profile of PBMCs in patients with acute RSV or Influenza infection Refer to individual Series

2012-03-12 | E-GEOD-32140 | biostudies-arrayexpress

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina]

2012-03-12 | GSE32139 | GEO

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Agilent].

2012-03-12 | GSE32138 | GEO

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina]

Project description:To investigate how human airway epithelial cells respond to Influenza or RSV infection, we harvested airway epithelial cells from the mainstream bronchi of human donors and cultured them as previously described (Pickles et al,1998) in a polarized system that resembles the in vivo mucociliary pseudostratified epithelium. Quadruplicate hAEC cultures were infected with 2X105 PFUs Influenza A (Udorn) or with 1x106 PFUs RSV for 2h or mock inoculated and harvested 24h after Influenza infection and 48h after RSV infection. Quadruplicate polarized airway epithelial cell cultures were infected with 2x10^5 PFUs of Influenza A (Udorn) for 2h or infected with 1x10^6 PFUs RSV and harvested 24 h post infection for Influenza or 48h post infection for RSV.Duplicate cultures were used as controls for each condition (Two cultures were mock treated mor 2h and harvested after 24h for the Influenza infection and 2 cultures were mock treated for 2h and harvested after 48 hours for the RSV infection.Total RNA was harvested and gene expression was studied using Genespring GX v7.3.1.

2012-03-12 | E-GEOD-32139 | biostudies-arrayexpress

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Agilent].

Project description:To investigate how human airway epithelial cells respond to Influenza or RSV infection, we harvested airway epithelial cells from the mainstream bronchi of human donors and cultured them as previously described (Pickles et al,1998) in a polarized system that resembles the in vivo mucociliary pseudostratified epithelium. Quadruplicate hAEC cultures were infected with 2X105 PFUs Influenza A (Udorn) or with 1x106 PFUs RSV for 2h or mock inoculated and harvested 24h after Influenza infection and 48h after RSV infection. Quadruplicate polarized airway epithelial cell cultures were mock treated or infected with 2x10^5 PFUs of Influenza A (Udorn) for 2h or infected with 1x10^6 PFUs RSV and harvested 24 h post infection for Influenza or 48h post infection for RSV. Total RNA was harvested and gene expression was studied using Genespring GX v7.3.1.

2012-03-12 | E-GEOD-32138 | biostudies-arrayexpress

Adaptation of influenza A (H7N9) virus in primary human airway epithelial cells.

Project description:Influenza A (H7N9) is an emerging zoonotic pathogen with pandemic potential. To understand its adaptation capability, we examined the genetic changes and cellular responses following serial infections of A (H7N9) in primary human airway epithelial cells (hAECs). After 35 serial passages, six amino acid mutations were found, i.e. HA (R54G, T160A, Q226L, H3 numbering), NA (K289R, or K292R for N2 numbering), NP (V363V/I) and PB2 (L/R332R). The mutations in HA enabled A(H7N9) virus to bind with higher affinity (from 39.2% to 53.4%) to sialic acid ?2,6-galactose (SA?2,6-Gal) linked receptors. A greater production of proinflammatory cytokines in hAECs was elicited at later passages together with earlier peaking at 24 hours post infection of IL-6, MIP-1?, and MCP-1 levels. Viral replication capacity in hAECs maintained at similar levels throughout the 35 passages. In conclusion, during the serial infections of hAECs by influenza A(H7N9) virus, enhanced binding of virion to cell receptors with subsequent stronger innate cell response were noted, but no enhancement of viral replication could be observed. This indicates the existence of possible evolutional hurdle for influenza A(H7N9) virus to transmit efficiently from human to human.

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Respiratory Syncytial virus (RSV)

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-05-29 | GSE232687 | GEO

Response of primary human airway epithelial cells to influenza infection: a quantitative proteomic study.

Project description:Influenza A virus exerts a large health burden during both yearly epidemics and global pandemics. However, designing effective vaccine and treatment options has proven difficult since the virus evolves rapidly. Therefore, it may be beneficial to identify host proteins associated with viral infection and replication to establish potential new antiviral targets. We have previously measured host protein responses in continuously cultured A549 cells infected with mouse-adapted virus strain A/PR/8/34(H1N1; PR8). We here identify and measure host proteins differentially regulated in more relevant primary human bronchial airway epithelial (HBAE) cells. A total of 3740 cytosolic HBAE proteins were identified by 2D LC-MS/MS, of which 52 were up-regulated ?2-fold and 41 were down-regulated ?2-fold after PR8 infection. Up-regulated HBAE proteins clustered primarily into interferon signaling, other host defense processes, and molecular transport, whereas down-regulated proteins were associated with cell death signaling pathways, cell adhesion and motility, and lipid metabolism. Comparison to influenza-infected A549 cells indicated some common influenza-induced host cell alterations, including defense response, molecular transport proteins, and cell adhesion. However, HBAE-specific alterations consisted of interferon and cell death signaling. These data point to important differences between influenza replication in continuous and primary cell lines and/or alveolar and bronchial epithelial cells.

| S-EPMC3411195 | biostudies-literature

MiRNA profiling in lung epithelial cells during infection with influenza A virus

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-01 | E-GEOD-44455 | biostudies-arrayexpress

Regional idiopathic pulmonary fibrosis airway epithelial cultures; Primary airway epithelial cells co-cultured with human lung fibroblasts

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-01 | GSE205527 | GEO