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The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

ABSTRACT: To confirm the function of the GAMYB homologs for Selaginella moellendorffii and Physcomitrella patens in rice cells, we examined the expression profile in the anthers of transgenic plants, which OsGAMYB, SmGAMYB, or PpGAMYB2 is expressed under the control of the rice GAMYB promoter in rice gamy mutant.

ORGANISM(S): Oryza sativa Oryza sativa Japonica Group

PROVIDER: GSE32652 | GEO | 2011/11/07

SECONDARY ACCESSION(S): PRJNA146963

REPOSITORIES: GEO

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The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

Project description:To confirm the function of the GAMYB homologs for Selaginella moellendorffii and Physcomitrella patens in rice cells, we examined the expression profile in the anthers of transgenic plants, which OsGAMYB, SmGAMYB, or PpGAMYB2 is expressed under the control of the rice GAMYB promoter in rice gamy mutant. Expression profiling was conducted using rice 4x44K microarray RAP-DB (G2519F#15241). The microarray was used to profile gene expression in OsGAMYB:OsGAMYB, OsGAMYB:SmGAMYB, OsGAMYB:PpGAMYB2 and vector transgenic plants. To assess the reproducibility of the microarray analysis, each microarray experiment was performed twice using RNA samples that had been isolated independently (i.e., two biological replicates), and included a dye-swapping test as a technical replicate for each biological replicate.

2011-11-07 | E-GEOD-32652 | biostudies-arrayexpress

Oryza sativa Japonica Group

Project description:The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

| PRJNA146963 | ENA

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Project description:To obtain the gene expression profiles of the moss Physcomitrella patens ABA insensitive mutant AR7, we performed microarray analysis of wildtype plant and AR7 using a custom Physcomitrella oligonucleotide microarray, which carries probes for 33,942 gene models of Physcomitrella genome version 1.1. on a 4 x 44 K Agilent platform.

2015-10-22 | GSE68914 | GEO

RNA sequencing of Nipponbare control and OsCNGC4, OsCNGC5 double knockout mutant in mature anthers

Project description:We created a double loss-of-function/knockout mutant targeting two rice genes simultaneously. The selected genes are as follows: OsCNGC4(LOC_Os03g44440) and OsCNGC5(LOC_Os12g28260). These two CNGCs are strongly transcriptional expressed in the rice mature anthers (stages 13-14). The mutant of these OsCNGC4/5 displayed a low seed-setting rate. This data refers to the transcriptome of mature anthers from the double mutant of OsCNGC4 and OsCNGC5. We sampled mature anther for the analysis.

2024-04-30 | E-MTAB-13943 | biostudies-arrayexpress

Genome-wide expression profiling of PpABI1 disruptants

Project description:To investigate the disruption effect of Group A PP2C on global gene expression in Physcomitrella patens, we performed microarray analysis of wildtype plant and PpABI1 disruptants using a custom Physcomitrella oligonucleotide microarray, which carries probes for 33,942 gene models of Physcomitrella genome version 1.1. on a 4 x 44 K Agilent platform.

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RNA-Seq of wild type and osmtd2 mutant rice anthers

Project description:Rice anthers at anthesis stage from the wild type and osmtd2 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

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RNA-Seq of wild type and MADS triple mutant anthers

Project description:Rice anthers at anthesis stage from the wild type and MADS triple mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2022-01-01 | E-MTAB-11246 | biostudies-arrayexpress

small RNA-seq of Physcomitrella patens rdr mutants

Project description:To investigate the effects of rdr2 and rdr6 mutations upon small RNAs in Physcomitrella patens , small RNA-seq was performed.

2015-06-30 | GSE51416 | GEO

Small RNAs from the moss Physcomitrella patens

Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing

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