Project description:Global transcriptional responses of shigella flexneri to RNA poly merase inhibitor We used two drugs with two concentration. At each concentration, there 3 time points.
Project description:Transcriptional program of a WT and ipaD strain of Shigella flexneri modeling the off- and on-state of the T3SA in vitro were compared by RNA-Seq
Project description:The genome-wide analysis was carried out to find out the genes responsible for single and multiple stress in Shigella flexneri 2a str. 2457T. This result can be offered to explain bacterial responses, such as the capacity to survive and elicit infections.
Project description:To explore what important role of PhoPQ TCS plays in Shigella virulence, the Agilent microarray technologies was used to compare the transcriptional profiles of Shigella flexneri 2a 301 and △phoPQ mutant strains at middle-log phase (6 h) or early-stationary phase (10 h) under LB growth conditions.
Project description:Analyse the transcriptionnal changes of human colon epithelial cells (HCT116) associated with Shigella flexneri (M90T) infection and correlated with the bacterial transcriptional program.
Project description:We evaluated the transcriptome changes induced by infection of Hela 229 cells with Shigella flexneri. The sample set consists of a control (mock), total population of infected sample and infected sample sorted into Shigella positive and Shigella negative population.
Project description:The genome-wide analysis were carried out to find out the genes reponsible for single and multiple stress in Shigella flexneri 2a str. 2457T. This results can be offered to explain bacterial responses such as the capacity of survive and elicit infections.
Project description:Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet the closely-related Shigella sonnei is replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model of Shigella infection, we discover that S. sonnei is more virulent than S. flexneri in vivo. Whole animal dual-RNAseq and testing of bacterial mutants suggest that S. sonnei virulence depends on its O-antigen oligosaccharide (which is unique among Shigella species). We show in vivo using zebrafish and ex vivo using human neutrophils that S. sonnei O-antigen can mediate neutrophil tolerance. Consistent with this, we demonstrate that O-antigen enables S. sonnei to resist phagolysosome acidification and promotes neutrophil cell death. Chemical inhibition or promotion of phagolysosome maturation respectively decreases and increases neutrophil control of S. sonnei and zebrafish survival. Strikingly, larvae primed with a sublethal dose of S. sonnei are protected against a secondary lethal dose of S. sonnei in an O-antigen-dependent manner, indicating that exposure to O-antigen can train the innate immune system against S. sonnei. Collectively, these findings reveal O-antigen as an important therapeutic target against bacillary dysentery, and may explain the rapidly increasing S. sonnei burden in developing countries.
Project description:In most eukaryotes and bacteria, queuosine (Q) replaces the guanosine at the wobble position of tRNAs harboring a GUN anticodon. To faithfully detect Q-modification in RNAs from Schizosaccharomyces pombe and Shigella flexneri, Q-MaP-Seq was established and applied to tRNAs from S. pombe WT (AEP1) cells and Shigella flexneri WT cells and tgt∆ cells. Q-modification of in vitro-transcribed RNAs and RNAs isolated from S. pombe and S. flexneri followed by reverse transcription using the RT-active DNA polymerase variant RT-KTq I614Y and sequencing of unmodified compared to modified RNAs allowed identification of Q-sites within tRNAs.