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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE34172 | GEO | 2012/01/26

SECONDARY ACCESSION(S): PRJNA149725

REPOSITORIES: GEO

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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.GR)

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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR)

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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge

Project description:This SuperSeries is composed of the following subset Series: GSE34158: Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.GR) GSE34159: Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR) GSE34160: Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR and EDTA) Refer to individual Series

2012-01-26 | E-GEOD-34172 | biostudies-arrayexpress

Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR and EDTA)

Project description:To determine differential gene expression in peripheral blood of asthmatic individuals undergoing allergen inhalation challenge, post-challenge compared to pre-challenge

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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (ER and DR)

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Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.GR)

Project description:Detecting differential changes in the peripheral whole-blood transcriptome, post-challenge compared to pre-challenge; using globin reduced PAXgene (PAX.GR) tubes Blood was collected immediately prior to, and two hours after challenge Blood samples from 4 subjects were used to study the effects of globin reduction on differential gene expression Preprocessing and filtering were applied to the PAX.GR dataset using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package in R was used to determine differential gene expression using a Benjamini Hochberg FDR of 5%.

2012-01-26 | E-GEOD-34158 | biostudies-arrayexpress

Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR)

Project description:Detecting differential changes in the peripheral whole-blood transcriptome, post-challenge compared to pre-challenge; using non-globin reduced PAXgene (PAX.NGR) tubes Blood was collected immediately prior to, and two hours after challenge Preprocessing and filtering were applied to the PAX.NGR dataset using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package in R was used to determine differential gene expression using a Benjamini Hochberg FDR of 5%.

2012-01-26 | E-GEOD-34159 | biostudies-arrayexpress

Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (PAX.NGR and EDTA)

Project description:To determine differential gene expression in peripheral blood of asthmatic individuals undergoing allergen inhalation challenge, post-challenge compared to pre-challenge Blood was collected immediately prior to, and two hours after challenge The 8 PAXgene non-globin reduced (PAX.NGR) samples were combined with 10 EDTA samples (n=9) for one analysis (PAX.NGR+EDTA) comparing gene expression in whole blood between pre and post challenge using the following covariates: tube type, sex, age, PC20 at pre-challenge, and FEV1 after onset and 2h post challenge and the RNA integrity number. Preprocessing and filtering were applied to the PAX.NGR+EDTA dataset using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package was used to determine differential gene expression using a Benjamini Hochberg FDR of 5%.

2012-01-26 | E-GEOD-34160 | biostudies-arrayexpress

Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (ER and DR)

Project description:To determine differential gene expression in peripheral blood of asthmatic individuals undergoing allergen inhalation challenge between early responders (ERs) and dual responders (DRs) following allergen inhalation challenge Blood was collected immediately prior to, and two hours after challenge The change in gene expression (post expression minus pre expression) in ERs was compared with the change in gene expression in DRs using age and sex as covariates Preprocessing and probeset filtering were applied to the entire dataset (28 CEL files) using the Factor Analysis for Robust MicroArray Summarization (farms) package in R The Linear Models for MicroArrays (limma) package was used to determine differential gene expression using a Benjamini Hochberg FDR of 10%.

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Expression data from peripheral blood - blood draws at Pre and Post time points of Methacholine (MCh) inhalation challenge

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