Gene expression profiles in Arabidopsis plants during the treatment of AgNO3.
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE34606 | GEO | 2011/12/22
SECONDARY ACCESSION(S): PRJNA150473
REPOSITORIES: GEO
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Project description:Camalexin, an indolic secondary metabolite, is the magor phytoalexin produced by Arabidopsis thaliana. Camalexin biosynthesis is induced by abiotic stresses such as heavy metal treatment (e.g AgNO3). With the aim of identifying positive regulators of camalexin biosynthesis in Arabidopsis, we listed putative transcription factor genes strongly induced during the time course of AgNO3 treatment from a transcriptome analysis. AgNO3 induced gene expression in Arabidopsis was measured at 48 and 72 hours after the 0.1 mM AgNO3 treatment. Arabidopsis seedlings were germinated under sterile conditions on GM-agar plates. Seedlings (one-week after germination) were then transferred to GM liquid culture and shaken for two weeks. Plants were then treated with 0.1 mM AgNO3 and sampled at 0 h, 48 h and 72 h after the treatment. Three independent experiments were performed at each time (0, 48, or 72 h) using different plants for each experiment.
2011-12-22 | E-GEOD-34606 | biostudies-arrayexpress
Project description:Indole-3-acetaldoxime (IAOx) represents an early intermediate of the biosynthesis of a variety of indolic secondary metabolites including the phytoanticipin indol-3-ylmethyl glucosinolate and the phytoalexin camalexin (3-thiazol-2′-yl-indole). Arabidopsis thaliana cyp79B2 cyp79B3 double knockout plants are completely impaired in the conversion of tryptophan to indole-3-acetaldoxime and do not accumulate IAOx-derived metabolites any longer. Consequently, comparative analysis of wild-type and cyp79B2 cyp79B3 plant lines has the potential to explore the complete range of IAOx-derived indolic secondary metabolites.
2012-05-22 | MTBLS2 | MetaboLights
Project description:Arabidopsis thaliana genes MLO2 (Mildew resistance locus-O 2), MLO6 and MLO12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. We show that early chlorosis and necrosis of rosette leaves in mlo2 mlo6 mlo12 mutants reflects an authentic but untimely leaf senescence program. Comparative transcriptional profiling revealed that transcripts of several genes encoding tryptophan/indole biosynthetic enzymes hyper-accumulate during vegetative development in the mlo2 mlo6 mlo12 mutant. Elevated expression levels of these genes correlate with altered steady-state levels of several indolic metabolites, including the phytoalexin camalexin and indolic glucosinolates, during development in the mlo2 single and the mlo2 mlo6 mlo12 triple mutant. Results of genetic epistasis analysis suggest a decisive role for indolic metabolites in mlo2-conditioned antifungal defense against both biotrophic powdery mildews and a camalexin-sensitive strain of the necrotrophic fungus, Botrytis cinerea. The wound- and pathogen-responsive callose synthase Powdery mildew resistance 4/Glucan-synthase-like 5 (PMR4/GSL5) was found to be responsible for the spontaneous callose deposits in mlo2 mutant plants but dispensable for mlo2-conditioned penetration resistance. Our data strengthen the notion that powdery mildew resistance of mlo2 genotypes is based on the same defense execution machinery as innate antifungal immune responses that restrict invasion of non-adapted fungal pathogens. Mature rosette leaves from WT and mlo2 mlo6 mlo12 triple mutant plants were harvested at 5, 6 and 7 weeks after sowing. 6 arrays.
2010-01-03 | E-GEOD-17875 | biostudies-arrayexpress
Project description:Arabidopsis thaliana genes MLO2 (Mildew resistance locus-O 2), MLO6 and MLO12 exhibit unequal genetic redundancy with respect to the modulation of defense responses against powdery mildew fungi and the control of developmental phenotypes such as premature leaf decay. We show that early chlorosis and necrosis of rosette leaves in mlo2 mlo6 mlo12 mutants reflects an authentic but untimely leaf senescence program. Comparative transcriptional profiling revealed that transcripts of several genes encoding tryptophan/indole biosynthetic enzymes hyper-accumulate during vegetative development in the mlo2 mlo6 mlo12 mutant. Elevated expression levels of these genes correlate with altered steady-state levels of several indolic metabolites, including the phytoalexin camalexin and indolic glucosinolates, during development in the mlo2 single and the mlo2 mlo6 mlo12 triple mutant. Results of genetic epistasis analysis suggest a decisive role for indolic metabolites in mlo2-conditioned antifungal defense against both biotrophic powdery mildews and a camalexin-sensitive strain of the necrotrophic fungus, Botrytis cinerea. The wound- and pathogen-responsive callose synthase Powdery mildew resistance 4/Glucan-synthase-like 5 (PMR4/GSL5) was found to be responsible for the spontaneous callose deposits in mlo2 mutant plants but dispensable for mlo2-conditioned penetration resistance. Our data strengthen the notion that powdery mildew resistance of mlo2 genotypes is based on the same defense execution machinery as innate antifungal immune responses that restrict invasion of non-adapted fungal pathogens.
2010-01-04 | GSE17875 | GEO
Project description:Arabidopsis thaliana efficiently synthesizes the antifungal phytoalexin camalexin without apparent release of bioactive intermediates, such as indole-3-acetaldoxime, suggesting channeling of the biosynthetic pathway involving an enzyme complex. To identify such protein interactions, two independent untargeted co-immunoprecipitation (Co-IP) approaches with the biosynthetic enzymes CYP71B15 and CYP71A13 as baits were performed and the camalexin biosynthetic P450 enzymes were co-purified. These interactions were confirmed by targeted Co-IP and förster resonance energy transfer measurements based on fluorescence lifetime imaging (FLIM-FRET). Furthermore, interaction of CYP71A13 and Arabidopsis P450 reductase 1 (ATR1) and recruitment of cytosolic gamma-glutamyl peptidase 1 (GGP1) to the endoplasmic reticulum (ER) was observed. An increased substrate affinity of CYP79B2 in presence of CYP71A13 was shown, indicating substrate channeling by the complex. During camalexin biosynthesis, indol-3-acetonitrile (IAN) is activated by CYP71A13 and glutathionylated to GS-IAN. To clarify whether this is mediated by a specific glutathione transferase (GST), CYP71A13 was expressed together with each of the 54 Arabidopsis GSTs in S. cerevisiae and GS-IAN-synthesis was observed for GSTU2. Corresponding studies of knock-out and overexpression plants did not show an altered camalexin content. However, an influence of the closely related GSTU4 on camalexin biosynthesis was demonstrated. GSTU4 is co-expressed with tryptophan- and camalexin specific enzymes and physically interacts with CYP71A13. Surprisingly, camalexin concentrations were reduced in knock-out and elevated in GSTU4 overexpressing plants. This shows that GSTU4 is not directly involved in camalexin biosynthesis but rather has a regulatory role or is involved in transport or a competing mechanism.
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Project description:We analyzed transcriptome changes from germinating cyp79B2 cyp79B3, qko, and pad3 mutant Arabidopsis seeds. Here were used mutant lines in Col-0 genetic background: a cyp79B2 cyp79B3 double mutant defective in indolic glucosinolate (cyp79B2/B3), a cyp79B2 cyp79B3 myb28 myb29 quadruple mutant defective in indolic and aliphatic glucosinolates (qko), and a camalexin deficient mutant (pad3). Differentially expressed genes were identified at 3, 6 and 10 days after sowing from both the mutant line seeds and from Alternaria brassicicola when inoculated seeds were analyzed.
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Project description:Apart from their primordial role in protein synthesis, tRNAs can be cleaved to produce tRNA-derived small RNAs (tsRNAs). The biological functions of tsRNAs in plants remain largely unknown. In this study, we developed RtcB-based RNA sequencing, a method that captures and distinguishes between 2’,3’-cyclic-phosphate (cP)-terminated RNAs and 3’-OH-terminated RNAs, and profiled 5’ tsRNAs and 5’ tRNA halves in Arabidopsis thaliana. We found that Arabidopsis 5’ tsRNAs and 5’ tRNA halves predominantly contain a cP at the 3’ end and require S-like RNase 1 (RNS1) and RNS3 for their production. One of the most abundant 5’ tsRNA, 5’ tsR-Ala, likely by associating with AGO1, negatively regulates Cytochrome P450 71A13 (CYP71A13) expression and camalexin biosynthesis to repress anti-fungal defense. 5’ tsR-Ala is downregulated upon fungal infection. Our study provides a global view of 5’ tsRNAs and 5’ tRNA halves in Arabidopsis and unravels an important role of a 5’ tsRNA in regulating anti-fungal defense.
2022-02-16 | GSE183560 | GEO
