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The NIH Human Pluripotent Stem Cell Database (Illumina, snp)

ABSTRACT: Genotyping was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Seventeen undifferentiated human embryonic stem cell lines and 11 human induced pluripotent stem cells were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35157 | GEO | 2012/10/31

SECONDARY ACCESSION(S): PRJNA155975

REPOSITORIES: GEO

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The NIH Human Pluripotent Stem Cell Database (Illumina, snp)

Project description:Genotyping was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Seventeen undifferentiated human embryonic stem cell lines and 11 human induced pluripotent stem cells were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov. 17 samples: 17 human ESC (UNDIFFerentiated) lines

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Project description:To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.

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