Transcriptomics

Dataset Information

0

Gene Expression Profiling Following DNA Vaccination of Rainbow Trout against Infectious Hematopoietic Necrosis Virus


ABSTRACT: The Infectious Hematopoietic Necrosic Virus (IHNV) DNA vaccine is based on the viral glycoprotein gene (G gene) and induces a non-specific anti-viral immune response and long-term specific immunity against IHNV in salmonid fishes. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine. Gene expression at the I.M. site was evaluated using a 16,000 feature salmon cDNA microarray. Eighty different transcripts were significantly modulated in the vector DNA group while 910 transcripts were modulated in the IHNV DNA vaccinated group relative to control group. Quantitative reverse transcriptase PCR was used to examine expression of selected genes at the I.M. site and in other secondary tissues. In the localized response (I.M. site), the magnitudes of gene expression changes were higher in the vaccinate group relative to the vector DNA group for the majority of genes analyzed. At secondary systemic sites (e.g. gill, kidney and spleen) evaluated with RT-PCR of specific genes, the main difference was the up-regulation of the type I IFN-related genes in only the IHNV DNA vaccinated group. Keywords: disease vaccine response

ORGANISM(S): Salmo salar Oncorhynchus mykiss

PROVIDER: GSE3522 | GEO | 2005/12/09

SECONDARY ACCESSION(S): PRJNA93571

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2005-12-09 | E-GEOD-3522 | biostudies-arrayexpress
2018-01-10 | GSE107116 | GEO
2015-04-01 | E-GEOD-56487 | biostudies-arrayexpress
2023-05-28 | GSE228111 | GEO
2023-05-28 | GSE227648 | GEO
2014-05-17 | E-GEOD-47057 | biostudies-arrayexpress
2023-05-28 | GSE228594 | GEO
2015-08-10 | E-GEOD-65241 | biostudies-arrayexpress
2012-07-18 | E-GEOD-31591 | biostudies-arrayexpress
2015-08-10 | GSE65241 | GEO