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Gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

OTHER RELATED OMICS DATASETS IN: PRJNA151581PRJNA155961PRJNA155777PRJNA152869PRJNA155779PRJNA155963

ORGANISM(S): Mus musculus

PROVIDER: GSE35284 | GEO | 2012/04/02

SECONDARY ACCESSION(S): PRJNA151581

REPOSITORIES: GEO

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Pulmonary miRNA expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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Pulmonary gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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Project description:We investigated hepatic mRNA profiles of female mice exposed to 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to this dose 1, 3 and 28 days after exposure, alongside respective controls. We show that genes part of the 3-hydroxy-3-methyl-glutaryl-CoA reductase pathway to be up-regulated by exposure to Printex 90. individual total RNA (200 ng) from 6 mice per treatment group (control and 0.162mg) and universal reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double stranded cDNA.

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Pulmonary miRNA expression in C57BL/6 non-pregnant female mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:miRNAs were measured in mice exposed to vehicle control (0.9% NaCl MilliQ water containing 10% v/v acellular BAL) or 0.162 mg Printex 90 in vehicle by intratracheal instillation and sacrificed 1, 3 and 28 days after exposure.

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Cardiac gene and miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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