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Microarray analysis of wildtype and Klf1-/- E13.5 fetal liver

ABSTRACT: The aim of this experiment was to investigate the role of KLF1 in the fetal liver Affymetrix microarrays were performed on fetal liver cells from E13.5 wildtype and Klf1-/- mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE35594 | GEO | 2012/02/07

SECONDARY ACCESSION(S): PRJNA152509

REPOSITORIES: GEO

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Microarray analysis of wildtype and Klf1-/- E13.5 fetal liver

Project description:The aim of this experiment was to investigate the role of KLF1 in the fetal liver Affymetrix microarrays were performed on fetal liver cells from E13.5 wildtype and Klf1-/- mice. Three wildtype replicates and three Klf1-/- replicates, all from E13.5 fetal liver.

2012-02-07 | E-GEOD-35594 | biostudies-arrayexpress

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Project description:Microarray analysis of wildtype and Klf1-/- E13.5 fetal liver

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KLF1/EKLF regulatory networks in primary erythroid cells

Project description:KLF1 (EKLF) regulates a diverse suite of genes to direct erythroid cell differentiation from bi-potent progenitors. To determine the local cis-regulatory contexts and transcription factor networks in which KLF1 operates, we performed KLF1 ChIP-seq in the mouse. We found at least 945 sites in the genome of E14.5 fetal liver erythroid cells which are occupied by endogenous KLF1. Many of these recovered sites reside in erythroid gene promoters such as β-globin, but the majority are distant to any known gene. Our data suggests KLF1 directly regulates most aspects of terminal erythroid differentiation including production of α and β-globin protein chains, heme biosynthesis, co-ordination of proliferation and anti-apoptotic pathways, and construction of the red cell membrane and cytoskeleton by functioning primarily as a transcriptional activator. Additionally, we suggest new mechanisms for KLF1 co-operation with other transcription factors, in particular the erythroid transcription factor GATA1, to maintain homeostasis in the erythroid compartment. Examination of KLF1 occupancy in primary erythroid cells. KLF1-ChIP and input samples were run on AB SOLiD Systems 2.0 and 3.0. The genomic alignment files (*sorted.txt) and peak file (*bed) contain the combined System 2.0 and 3.0 data.

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Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

Project description:The aim of this experiment was to investigate the role of KLF3 in regulating gene expression at different stages throughout the erythroid maturation process. Affymetrix microarrays were performed on fetal liver cells (both TER119- progenitor cells and TER119+ erythroblast cells) from E14.5 wildtype and Klf3 KO mice. Four wildtype TER119- replicates, four Klf3 KO TER119- replicates, four wildtype TER119+ replicates, three Klf3 KO TER119+ replicates. All are from E14.5 fetal liver.

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Microarray analysis of wildtype and Klf3 KO E14.5 fetal liver cells (TER119- and TER119+)

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