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Functional inactivation of Thyroid Transcription Factor-1 in PCCl3 thyroid cells

ABSTRACT: Thyroid Transcription Factor-1 (TTF-1) is a key regulator of thyroid development and function. In order to identify the genes whose expression depends on TTF-1 transcriptional activity within the thyrocyte we analyzed the consequence of the functional inactivation of this factor in PCCl3 cells. The expression of a fusion protein composed of the DNA binding domain of TTF-1 and of the strong repressive domain of the Engrailed protein (Engr HD) resulted in a dramatic loss of epithelial cell morphology and in proliferation arrest. These changes were reversed when the inhibition of endogenous TTF-1 was relieved. No change was observed when a similar fusion protein containing point mutations abolishing DNA binding activity (Engr HDm) was produced in the cells.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE35623 | GEO | 2012/07/01

SECONDARY ACCESSION(S): PRJNA152463

REPOSITORIES: GEO

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