Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A panel of hepatocellular carcinoma cell lines recapitulates the molecular subtypes of the human disease: A model for targeted drug development

ABSTRACT: RNA expression patterns of liver cell lines were compared to those of liver tumors to determine the suitability of cell lines as a model for drug development studies. The cell lines appear to recapitulate liver tumors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE35818 | GEO | 2012/02/15

SECONDARY ACCESSION(S): PRJNA152151

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

A panel of hepatocellular carcinoma cell lines recapitulates the molecular subtypes of the human disease: A model for targeted drug development

Project description:RNA expression patterns of liver cell lines were compared to those of liver tumors to determine the suitability of cell lines as a model for drug development studies. The cell lines appear to recapitulate liver tumors. Gene expression of 20 individual liver cell lines relative to a liver cell line reference mix containing equal amounts of 19 liver cell lines. The expression data was correllated with expression in liver tumors.

2012-02-15 | E-GEOD-35818 | biostudies-arrayexpress

Mouse Smarcb1-deficient models recapitulate subtypes of human rhabdoid tumors.

Project description:Mouse Smarcb1-deficient models recapitulate subtypes of human rhabdoid tumors.

| PRJNA269772 | ENA

Targeted RNA Sequencing of a panel of ovarian cancer cell lines

Project description:Assess the baseline expression of the genes in the Oncology Biomarker Panel across the panel of ovarian and cervical cancer cell lines. This data will then be compared to the sensitivity of the cell lines to DDR inhibitors. The expression data will be correlated with the sensitivity of the cell lines to see whether determinants of sensitivity described in the literature translate. A hierarchy of genes will be determined. It is expected that the cells that are more sensitive to DDR inhibitors will have decreased expression of the genes known to be determinants of sensitivity.

2020-05-21 | GSE150942 | GEO

Cytogenetic aberrations in hepatocellular carcinoma [II]

Project description:Array CGH (44K) of liver tumors and unaffected liver controls from mice conditionally deficient for Mad2 and p53 in hepatocytes aCGH of sample DNA was hybridized with sex mismatched control DNA from a different mouse. Tumor and unaffected liver tissues were tested for each mouse. 19 tissue samples, 10 tumors, 9 normal samples

2015-11-01 | E-GEOD-63065 | biostudies-arrayexpress

Re-expression of fetal IGF2 as a target for hepatocellular carcinoma therapy

Project description:Non-coding microRNAs (miRNAs) mainly regulate the expression of targeted genes by regulating mRNA degradation or repressing their protein translation. MiRNA microarray profiling was then performed on 218 human HCC tumors samples, 10 samples from adjacent cirrhotic non-tumoral tissue, 10 samples from healthy liver and 12 HCC cell lines. In this study we investigated which miRNAs were differentially expressed in HCC compared to cirrhotic non-tumoral tissue and healthy liver.

2016-11-03 | GSE74618 | GEO

Cytogenetic aberrations in hepatocellular carcinoma [I]

Project description:Array CGH (44K) of liver tumors and unaffected liver controls from mice conditionally deficient for Mad2 and p53 in hepatocytes

2015-11-01 | GSE63064 | GEO

Cytogenetic aberrations in hepatocellular carcinoma [II]

Project description:Array CGH (44K) of liver tumors and unaffected liver controls from mice conditionally deficient for Mad2 and p53 in hepatocytes

2015-11-01 | GSE63065 | GEO

Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance

Project description:Current preclinical models in tumor biology are limited in their ability to recapitulate relevant (patho-) physiological processes, including autophagy. Three-dimensional (3D) growth cultures have frequently been proposed to overcome the lack of correlation between two-dimensional (2D) monolayer cell cultures and human tumors in preclinical drug testing. Besides 3D growth, it is also advantageous to simulate shear stress, compound flux and removal of metabolites, e.g. via bioreactor systems, through which culture medium is constantly pumped at a flow rate reflecting physiological conditions. Here, we show that both Staticic 3D growth and 3D growth within a bioreactor system modulate key hallmarks of cancer cells, including proliferation and cell death as well as macroautophagy, a recycling pathway often activated by highly proliferative tumors to cope with metabolic stress. The autophagy-related gene expression profiles of 2D- and 3D-grown cells are substantially different, with the 3D-grown cells exhibiting an expression profile closely resembling the (patho-) physiological Statice of a tumor. Underscoring the importance of this pathway, autophagy-controlling transcription factors, such as TFEB and FOXO3, are upregulated in tumors, and 3D-grown cells have increased expression compared with cells grown in 2D conditions. Three-dimensional cultures depleted of the autophagy mediators BECN1, ATG5 or ATG7 or the transcription factor FOXO3, are more sensitive to cytotoxic treatment. Accordingly, combining cytotoxic treatment with compounds affecting late autophagic flux, such as chloroquine, renders the 3D-grown cells more susceptible to therapy and increases intracellular doxorubicin concentration to the level of 2D-grown cells. Altogether, 3D cultures are a valuable tool to study drug response of tumor cells, as these models recapitulate (patho-) physiologically relevant pathways, such as autophagy.

2017-07-04 | GSE100020 | GEO

Eastern Asian expert panel opinion: designing clinical trials of molecular targeted therapy for hepatocellular carcinoma.

Project description:The largest burden of hepatocellular carcinoma (HCC) lies in Asia, secondary to hepatitis B virus (HBV) infection. Improved survival with sorafenib has fostered new research but many challenges remain in designing clinical trials. The disease, its management, and populations affected by it are heterogeneous worldwide and within Asia. An expert conference of Eastern Asian oncologists and hepatologists was convened to foster consensus in clinical trial design. The panel identified key areas that need to be addressed to facilitate clinical trials in Asia. Stratification by viral etiology is desirable within Asia and by region in global trials. Antiviral therapy should also be considered as a stratification factor and incorporated into HCC management in trials. The panel agreed that histological diagnosis is not required for trial entry and that Barcelona-Clinic Liver Cancer (BCLC) staging is acceptable for trials as long as portal hypertension can be better defined with standardized methodology. Consensus in treatment must be sought to allow multi-national trials and it must be recognized that first-line sorafenib is not largely feasible in Asia. Finally, Asian nations must be urged to participate in clinical trials, many of which are ongoing, to advance new treatment options in this challenging disease.

| S-EPMC2989333 | biostudies-literature

Cytogenetic aberrations in Hepatocellular adenoma and carcinoma [III]

Project description:array CGH (244K) of liver tumors and unaffected liver controls from mice conditionally deficient for Mad2 and p53 in hepatocytes aCGH of sample DNA was hybridized with sex mismatched control DNA from a different mouse. Tumor and unaffected liver tissues were tested for each mouse. 19 tissue samples, 10 tumors, 9 normal samples

2015-11-01 | E-GEOD-63066 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data