Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Digitoxin treatment versus control in HEK293 cells

ABSTRACT: We treated HEK293 cells with the cardiotonic steroid digitoxin to identify alternative splicing changes as compared to DMSO treatment alone.

ORGANISM(S): Homo sapiens

PROVIDER: GSE36058 | GEO | 2012/05/01

SECONDARY ACCESSION(S): PRJNA151805

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Digitoxin treatment versus control in HEK293 cells

Project description:We treated HEK293 cells with the cardiotonic steroid digitoxin to identify alternative splicing changes as compared to DMSO treatment alone. In this set, 6 total samples were analyzed. There were three biological replicates for each of two conditions: DMSO treatment and Digitoxin treatment. Arrays were analyzed for gene expression using GeneBase and for alternative splicing using MADS+ and OmniViewer software.

2012-04-30 | E-GEOD-36058 | biostudies-arrayexpress

shRNA mediated knockdown of TRA2B and SRSF3 in HEK293 cell line

Project description:We have independently knocked down TRA2B and SRSF3 in HEK293 cells to identify the sum of alternative splicing changes that results from depletion of each factor. Each was compared to a control transfection of the hairpin vector alone. In this set, 9 total samples were analyzed. There were three biological replicates for each of three conditions: knockdown control, TRA2B knockdown and SRSF3 knockdown. Arrays were analyzed for gene expression using GeneBase and for alternative splicing using MADS+ and OmniViewer software.

2012-04-30 | E-GEOD-36069 | biostudies-arrayexpress

shRNA mediated knockdown of TRA2B and SRSF3 in HEK293 cell line

2012-05-01 | GSE36069 | GEO

Translational profiling of RBMX-overexpressed HEK293 cells

Project description:Wild-type and RBMX-overexpressed HEK293 cells where profiled at the transcriptome and translatome levels through polysomal profiling. Paired-end RNA-seq libraries were built and sequenced on an Illumina HiSeq 2000 machine to allow for accurate gene expression quantification and alternative splicing isoforms detection.

2019-12-01 | GSE68990 | GEO

The major and minor spliceosome interact to regulate alternative splicing [HEK293 cells]

Project description:The major and minor spliceosome interact to regulate alternative splicing [HEK293 cells]

| PRJNA634876 | ENA

Homo sapiens

Project description:Digitoxin treatment versus control in HEK293 cells

| PRJNA151805 | ENA

Cohesin regulates alternative splicing alone or with BRD4

Project description:Cohesin regulates alternative splicing alone or with BRD4

| PRJNA758423 | ENA

Characterization of co-transcriptional splicing in acute lymphoblastic leukemia

Project description:Alternative splicing is a promising therapeutic target in acute lymphoblastic leukemia. Here, we report the alternative splicing changes in B-ALL cell lines including NALM6 cells and MUTZ-5 cells treated with DYRK1 inhibitor, EHT 1610 and GNF 2133. Dysregulation of co-transcriptional splicing is a therapeutic target in acute lymphoblastic leukemia (ALL). Here, we detected the alternative splicing in NALM6 cells or MUTZ-5 cells treated with EHT 1610 or GNF2133. We detected the alternative splicing in NALM6 cells cells treated with THAL-SNS-032. We also determined the alternative splicing in MEF2D-BCL9 ALL and MEF2D-HNRNPUL1 ALL patient xenografted samples treated with E7820, a anticancer sulfamide, or DMSO. Furthermore, ChIP-seq for RNA polymerase II (Pol II), p-Ser2 Pol II, and p-Ser5 Pol II were performed after DMSO or EHT 1610 treatment in NALM6 cells. ChIP-seq for p-Ser2 Pol II, and p-Ser5 Pol II were performed after DMSO or THAL-SNS-032 treatment in NALM6 cells.PRO-seq in NALM6 cells treated with DMSO, EHT 1610, and THAL-SNS-032 were performed.

2024-09-09 | GSE252550 | GEO

Human PRPF39 is an alternative splicing factor recruiting U1 snRNP to weak 5' splice sites

Project description:To investigate the role of PRPF39 in the regulation of alternative splicing, we performed RNAseq and splicing analysis on PRPF39 knockdown and control HEK293 cells

2022-08-11 | GSE210544 | GEO

Starved HEK293 cells

Project description:Starved HEK293 cells

| PRJNA533129 | ENA