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Renal medullary genes in salt-sensitive hypertension

ABSTRACT: Substitution of chromosome 13 from Brown Norway BN/SsNHsd/Mcw (BN/Mcw) rats into the Dahl salt-sensitive SS/JrHsd/Mcw (SS/Mcw) rats resulted in substantial reduction of blood pressure salt sensitivity in this consomic rat strain designated SSBN13. In the present study, we attempted to identify genes associated with salt-sensitive hypertension by utilizing a custom, known-gene cDNA microarray to compare the mRNA expression profiles in the renal medulla (a tissue playing a pivotal role in long-term blood pressure regulation) of SS/Mcw and SSBN13 rats on either low-salt (0.4% NaCl) or high-salt (4% NaCl, 2 wk) diets. Keywords: Dahl S rat; blood pressure; kidney; consomic rats

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE3608 | GEO | 2005/11/17

SECONDARY ACCESSION(S): PRJNA93713

REPOSITORIES: GEO

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Renal medullary genes in salt-sensitive hypertension

Project description:Substitution of chromosome 13 from Brown Norway BN/SsNHsd/Mcw (BN/Mcw) rats into the Dahl salt-sensitive SS/JrHsd/Mcw (SS/Mcw) rats resulted in substantial reduction of blood pressure salt sensitivity in this consomic rat strain designated SSBN13. In the present study, we attempted to identify genes associated with salt-sensitive hypertension by utilizing a custom, known-gene cDNA microarray to compare the mRNA expression profiles in the renal medulla (a tissue playing a pivotal role in long-term blood pressure regulation) of SS/Mcw and SSBN13 rats on either low-salt (0.4% NaCl) or high-salt (4% NaCl, 2 wk) diets. To increase the reliability of microarray data, we designed a four-way comparison experiment incorporating several levels of replication and developed a conservative yet robust data analysis method. Using this approach, from the 1,751 genes examined (representing more than 80% of all currently known rat genes), we identified 80 as being differentially expressed in at least 1 of the 4 comparisons.

2010-06-30 | E-GEOD-3608 | biostudies-arrayexpress

Identification of gene modulating vascular inflammation using chromosome 2 fragment substitutions and RNA sequencing [miRNA-Seq]

Project description:Chromosome 2 introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt-sensitive (SS) background (consomic S2B) reduced blood pressure (BP) and vascular inflammation under normal salt diet (NSD). We hypothesized that BN chromosome 2 contains anti-inflammatory genes that could reduce BP elevation and vascular inflammation in rats fed NSD and high salt diet (HSD). We used chromosome 2 fragment substitutions to map chromosome 2 portion associated with vascular inflammation changes and next generation sequencing (NGS) to profile microRNAs in thoracic descending aorta of SS and congenic rats fed NSD or HSD.

2021-03-22 | GSE149515 | GEO

Identification of gene modulating vascular inflammation using chromosome 2 fragment substitutions and RNA sequencing [RNA-Seq]

Project description:Chromosome 2 introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt-sensitive (SS) background (consomic S2B) reduced blood pressure (BP) and vascular inflammation under normal salt diet (NSD). We hypothesized that BN chromosome 2 contains anti-inflammatory genes that could reduce BP elevation and vascular inflammation in rats fed NSD and high salt diet (HSD). We used chromosome 2 fragment substitutions to map chromosome 2 portion associated with vascular inflammation changes and next generation sequencing (NGS) to profile messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs) in thoracic descending aorta of SS and congenic rats fed NSD or HSD.

2021-03-22 | GSE149514 | GEO

Transcriptomic Analysis in Renal T Lymphocytes Exposes Sodium-Independent Dietary Differences in Dahl Salt-Sensitive Rats

Project description:Background. The Dahl salt-sensitive (SS) rat is an established model of salt-sensitive hypertension and renal damage. Recently, sodium-independent dietary effects were shown to be important in the development of the SS hypertensive phenotype. Compared to Dahl SS/JrHsdMcwi (SS/MCW) rats fed a purified diet (AIN-76A), grain-fed Dahl SS/JrHsdMcwiCrl rats (SS/CRL; Teklad 5L2F) were less susceptible to salt-induced hypertension and renal damage. Methods. With the known role of the immune system in hypertension, the present study characterized the immune cells infiltrating SS/MCW and SS/CRL kidneys. To further identify distinct molecular pathways between SS/MCW and SS/CRL, transcriptomic analysis was performed via RNA sequencing in T-cells isolated from the blood and kidneys of low and high salt-fed rats. Results. Following a 3-week high salt (4.0% NaCl) challenge, SS/CRL rats were protected from salt-induced hypertension (116.5±1.2 vs 141.9±14.4 mmHg) and albuminuria (21.7±3.5 vs 162.9±22.2 mg/day) compared to SS/MCW. Additionally, the absolute number of immune cells infiltrating the kidney was significantly reduced in SS/CRL. RNA-seq revealed >50% of all annotated genes in the entire transcriptome to be significantly differentially expressed in T-cells isolated from blood versus kidney. Pathway analysis of significant differentially expressed genes between SS/MCW and SS/CRL renal and circulating T-cells demonstrated salt-induced changes in genes related to inflammation in SS/MCW compared to metabolism-related pathways in SS/CRL. Conclusions. These functional and transcriptomic T-cell differences between SS/MCW and SS/CRL show that sodium-independent dietary effects may influence the immune response and infiltration of immune cells into the kidney, ultimately impacting susceptibility to salt-induced hypertension and renal damage.

2019-08-26 | GSE127000 | GEO

Transcription profiling of lung from Dahl salt sensitive (SS) rats and SS11BN consomic rats during hypoxia treatment

Project description:Examination of gene expression changes during hypoxia of the Dahl salt sensative (SS) rat and SS11BN consomic rat. SS11BN rats are derived from introgression of chromosome 11 from Brown Norway (BN) rat into the SS genetic background.

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Transcription profiling of rat lung response to hypoxia of the Dahl salt sensative (SS) rat and SS13BN cosomic rat SS13BN rats are derived from introgression of chromosome 13 from the Brown Norway (BN) rat into the SS genetic background

Project description:Examination of gene expression changes during hypoxia of the Dahl salt sensitive (SS) rat and SS13BN consomic rat. SS13BN rats are derived from introgression of chromosome 13 from Brown Norway (BN) rat into the SS genetic background.

2005-01-01 | E-TIGR-63 | biostudies-arrayexpress

Transcription profiling of liver response to hypoxia of the Dahl salt sensative (SS) rat and SS13BN cosomic rat SS13BN rats are derived from introgression of chromosome 13 from the Brown Norway (BN) rat into the SS genetic background

Project description:Examination of gene expression changes during hypoxia of the Dahl salt sensitive (SS) rat and SS13BN consomic rat. SS13BN rats are derived from introgression of chromosome 13 from the Brown Norway (BN) rat into the SS genetic background.

2005-01-01 | E-TIGR-62 | biostudies-arrayexpress

Transcription profiling of rat lung response to hypoxia of the Dahl salt sensitive (SS) rat and SS7BN consomic rat SS7BN rats are derived from introgression of chromosome 7 from the Brown Norway (BN) rat into the SS genetic background

Project description:Examination of gene expression changes during hypoxia of the Dahl salt sensitive (SS) rat and SS7BN consomic rat. SS7BN rats are derived from introgression of chromosome 7 from the Brown Norway (BN) rat into the SS genetic background.

2005-01-01 | E-TIGR-75 | biostudies-arrayexpress

Transcription profiling of rat kidney response to hypoxia of the Dahl salt sensitive (SS) rat and SS7BN consomic rat SS7BN rats are derived from introgression of chromosome 7 from the Brown Norway (BN) rat into the SS genetic background

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Transcription profiling of lung from Dahl salt sensitive (SS) rats and SS4BN consomic rats during hypoxia treatment

Project description:Examination of gene expression changes during hypoxia of the Dahl salt sensative (SS) rat and SS4BN consomic rat. SS4BN rats are derived from introgression of chromosome 4 from Brown Norway (BN) rat into the genetic background.

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