Transcriptomics

Dataset Information

0

Breast cancer cell lines treated with EZH2 inhibitor compounds


ABSTRACT: Loss of H3K27me3 repressive chromatin histone marks, maintained by the histone methyltransferase (HKMT) EZH2, may lead to reversal of epigenetic silencing in tumor cells and have therapeutic potential. Using a cell-based assay, we have identified three compounds from a HKMT inhibitor chemical library which re-express H3K27me3 mediated, silenced genes. Chromatin immunoprecipitation verified a decrease in silencing marks (H3K27me3, H3K9me3) and importantly an increase in active marks (H3K4me2/3, H3K27ac) at the promoter of re-expressed genes. Compound treated breast tumor cells induced enrichment for genome-wide changes in expression of known target genes for EZH2 and induced cell growth inhibition: with most sensitive breast tumor cell lines having low EZH2 protein expression, while a normal epithelial breast line was least sensitive.

ORGANISM(S): Homo sapiens

PROVIDER: GSE36131 | GEO | 2013/08/27

SECONDARY ACCESSION(S): PRJNA152943

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-08-27 | E-GEOD-36131 | biostudies-arrayexpress
2018-07-03 | PXD010073 | Pride
2013-06-20 | E-GEOD-45579 | biostudies-arrayexpress
2013-06-20 | GSE45579 | GEO
2018-11-21 | GSE110133 | GEO
2010-05-18 | E-GEOD-15890 | biostudies-arrayexpress
2013-10-25 | E-GEOD-50951 | biostudies-arrayexpress
2014-07-01 | E-GEOD-54029 | biostudies-arrayexpress
2009-06-02 | GSE15890 | GEO
2013-07-01 | GSE37321 | GEO