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Silencing or amplification of endocannabinoid signaling in blastocysts via CB1 compromises trophoblast cell migration

ABSTRACT: Endocannabinoid signaling plays a key role in multiple events in female reproduction. In this investigation, we discovered an interesting phenomenon that mice with either elevated or silenced endocannabinoid signaling show similar defects in multiple pregnancy events, including preimplantation embryo development. To unravel the underlying mechanisms, microarray studies were was conducted using RNAs collected from WT, Cnr1-/- and Faah-/- mouse blastocysts on day 4 of pregnancy. The results show that about 100 genes showed unidirectional changes under either elevated or silenced endocannabinoid signaling. Analysis of functional grouping of these genes revealed that multiple biological functions and pathways are affected under aberrant endocannabinoid signaling, including cell migration. Several genes from the microarray data were confirmed by quantitative RT-PCR. Cell motility assays validated the predicted compromised cell migration in Cnr1-/- and Faah-/- trophoblast stem cells. This study provides molecular basis for biphasic effects of endocannabinoid signaling in female reproduction

ORGANISM(S): Mus musculus

PROVIDER: GSE36399 | GEO | 2012/07/26

SECONDARY ACCESSION(S): PRJNA153291

REPOSITORIES: GEO

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Silencing or amplification of endocannabinoid signaling in blastocysts via CB1 compromises trophoblast cell migration

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