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Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221ΔFasX following exposure to human plasma

ABSTRACT: The fasX gene encodes a small regulatory RNA in the group A Streptococcus (GAS). To determine the FasX regulon during GAS exposure to human plasma we compared parental strain MGAS2221 with isogenic fasX mutant strain 2221ΔFasX. Gene expression was analyzed 0, 15, and 60 minutes post-plasma exposure.

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE36645 | GEO | 2012/08/01

SECONDARY ACCESSION(S): PRJNA153649

REPOSITORIES: GEO

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Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221ΔFasX following exposure to human plasma

Project description:The fasX gene encodes a small regulatory RNA in the group A Streptococcus (GAS). To determine the FasX regulon during GAS exposure to human plasma we compared parental strain MGAS2221 with isogenic fasX mutant strain 2221ΔFasX. Gene expression was analyzed 0, 15, and 60 minutes post-plasma exposure. GAS strains were grown in THY broth to mid-exponential phase, corresponding to an O.D.600 of 0.5. Two 16ml aliquots of each GAS culture were pelleted by centrifugation and the supernatant removed. Each GAS cell pellet was resuspended in 16ml of pre-warmed human plasma. Thus, four GAS-plasma suspensions were created, two each for each of the GAS strains, with one of the two using plasma from person A, and the other two using plasma from person B. Aliquots were recovered from each of the four GAS-plasma suspensions at T = 0, 15, and 60 mins. For the first two time-points 4.5ml of GAS-plasma suspension were added to 9ml of RNAprotect (Qiagen Inc.). For the final time-point, 4ml was added to 8ml of RNAprotect. Following addition of RNAprotect, samples were incubated at room temperature for 5 mins before centrifuging. Supernatant was removed and the pellets quick frozen in liquid nitrogen and stored at -80 °C until ready to use. RNA isolation, cDNA synthesis, and use of our custom Affymetrix microarray was as previously described (Perez et al., 2009)

2012-07-31 | E-GEOD-36645 | biostudies-arrayexpress

Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221ΔrivRX

2012-12-01 | GSE36646 | GEO

Transcriptome comparison of S. pyogenes strains MGAS2221 and 2221?rivRX

Project description:The rivR gene encodes a putative transcriptional regulator, while the rivX gene encodes a putative small regulatory RNA. To determine the RivRX regulon during exponential phase growth in THY broth we compared parental strain MGAS2221 with isogenic rivRX mutant strain 2221?rivRX. GAS strains were grown in THY broth to mid-exponential phase, corresponding to an O.D.600 of 0.5. A 4.5ml aliquot of each GAS culture was added to 9ml of RNAprotect (Qiagen Inc.). Following addition of RNAprotect, samples were incubated at room temperature for 5 mins before centrifuging. Supernatant was removed and the pellets quick frozen in liquid nitrogen and stored at -80 °C until ready to use. RNA isolation, cDNA synthesis, and use of our custom Affymetrix microarray was as previously described (Perez et al., 2009)

2012-12-01 | E-GEOD-36646 | biostudies-arrayexpress

Streptococcus pyogenes strain:MGAS2221

Project description:Streptococcus pyogenes strain:MGAS2221 Genome sequencing

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Streptococcus pyogenes strain:MGAS2221

Project description:Streptococcus pyogenes strain:MGAS2221 Genome sequencing

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Streptococcus pyogenes strain:MGAS2221

Project description:Streptococcus pyogenes strain:MGAS2221 Raw sequence reads

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The role of Stk in Streptococcus pyogenes

Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221

2011-08-01 | GSE28881 | GEO

Transcriptome comparison of Streptococcus pyogenes strain MGAS2221 with that of an isogenic PEL mutant

Project description:PEL is a small regulatory RNA that is present within the S. pyogenes genome. In some strains of S. pyogenes, the PEL sRNA regulates many of the known virulence factors produced by this pathogen. A genome-wide analysis of PEL-regulated genes had not been performed previously so we analyzed this by comparing the parental M1T1 isolate MGAS2221 with an isogenic PEL mutant. Comparing gene transcript levels between these two strains at the exponential and stationary phases of growth, we identified that PEL has no regulatory function in MGAS2221. Three cultures of each GAS strain were grown in THY broth to exponential (O.D. 0.5) and stationary (O.D. 1.7) phases of growth. Two volumes of RNA protect were added to samples recovered at these O.D.'s, the samples incubated at room temperature for 5 minutes, and the bacteria collected through centrifugation. Total RNA was isolated via a mechanical disruption method, converted to cDNA, fragmented, labeled, and hybridized to our Affymetrix microarray. Estimates of gene expression were calculated using GCOS software v1.4.

2009-08-25 | E-GEOD-17767 | biostudies-arrayexpress

A genome-wide analysis of small regulatory RNAs in the human pathogen group A Streptococcus

Project description:This SuperSeries is composed of the following subset Series: GSE17767: Transcriptome comparison of Streptococcus pyogenes strain MGAS2221 with that of an isogenic PEL mutant GSE17789: Tiling microarray-based identification of small regulatory RNAs in the group A Streptococcus (GAS) Refer to individual Series

2009-08-25 | E-GEOD-17790 | biostudies-arrayexpress

The role of Stk in Streptococcus pyogenes

Project description:Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221 The RNA prepared from triplicate cultures for the wild type and the stk mutant strains was each labeled with Cy3 and hybridized to duplicate arrays. Reference RNA consisted of pooled RNA for the wild type and stk mutant, labeled with Cy5.

2011-07-31 | E-GEOD-28881 | biostudies-arrayexpress

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