Transcriptomics

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BW25113 GhoS for RNase activity


ABSTRACT: To provide more evidence of the specificity of the RNase activity of GhoS, we performed a whole-transcriptome study for the production of GhoS vs. an empty plasmid so that we could investigate all of the cell’s transcripts for cleavage with GhoS, in vivo (i.e., BW25113/pCA24N-ghoS vs. BW25113/pCA24N with 1 mM IPTG induction of ghoS for 90 min). Under these conditions, only 20 genes were found to be repressed by more than 4-fold; there were no induced genes. These GhoS-repressed genes were all involved in the biosynthesis/transport of purines and pyrimidines; among them, pyrI was most highly repressed (-20 fold). These results suggest that GhoS selectively cleaves only a few cellular targets.

ORGANISM(S): Escherichia coli

PROVIDER: GSE36779 | GEO | 2012/08/30

SECONDARY ACCESSION(S): PRJNA156915

REPOSITORIES: GEO

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