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Gene expression profile after injection of recombinant deleted non-virion protein gene (NV) VHSV

ABSTRACT: The function of the non-virion protein (NV) of the Viral hemorrhagic septicemia virus (VHSV) has been long questioned but it still remains unknown. We report here the differences in gene expression profiles of trouts infected with deleted NV VHSV or wild type VHSV so that it could shed some light on the issue.

ORGANISM(S): Oncorhynchus mykiss

PROVIDER: GSE37330 | GEO | 2013/08/17

SECONDARY ACCESSION(S): PRJNA161027

REPOSITORIES: GEO

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Gene expression profile after injection of recombinant deleted non-virion protein gene (NV) VHSV

Project description:The function of the non-virion protein (NV) of the Viral hemorrhagic septicemia virus (VHSV) has been long questioned but it still remains unknown. We report here the differences in gene expression profiles of trouts infected with deleted NV VHSV or wild type VHSV so that it could shed some light on the issue. Eight fingerlings of rainbow trout were used for the experiment. Four of them were injected with deleted NV VHSV and the rest were injected with wild type VHSV as a control. Head kidney and spleen of each trout were collected 2 days post-injection and total RNA was extracted.

2013-08-17 | E-GEOD-37330 | biostudies-arrayexpress

Gene expression profile after injection of rhabdoviral recombinant protein NV [PBS vs. NV]

2014-05-05 | E-GEOD-37797 | biostudies-arrayexpress

Gene expression profile after injection of rhabdoviral recombinant protein NV [PBS vs. NV]

2014-05-05 | GSE37797 | GEO

Gene expression profile after injection of rhabdoviral recombinant protein NV

2014-08-11 | GSE31557 | GEO

Gene expression profile after injection of rhabdoviral recombinant protein NV

Project description:Due to the common presence of a non-virion NV gene absent from other fish rhabdoviruses, NV-coding rhabdoviruses, such as the viral haemorrhagic septicemia (VHSV), were placed into a new Novirhabdovirus genus. Because conflicting results do exist on the importance of NV for VHSV trout pathogenicity and the NV function is still unclear, the effects of intraperitoneal injection of recombinant NV protein were studied by using home-designed immune-related microarrays from rainbow trout Oncorhynchus mykiss. The results were compared with parallel experiments using bacterial flagellins, a well know mammalian agonist of toll receptors (tlr5) with adjuvant activity recently described also in fish Four experimental groups were formed with four biological replicates each, 16 samples in total. First one was injected with NV recombinant protein in PBS, second one was a control group injected with PBS, the third group was injected with bacterial flagellins in PBS and the fourth group was injected with polihistidine peptide as a reference group because the recombinant proteins have a polihistidine tail. There is a technical replicate within each array.

2014-08-11 | E-GEOD-31557 | biostudies-arrayexpress

Gene expression profiles after injection of zebrafish with NV (non virion) recombinant protein from Viral Hemorrhagic Septicemia Virus (VHSV)

Project description:NV is a viral protein only present in the genus Novirhabdovirus (fam Rhabdoviridae), its function is not well characterized yet. In this study we try to shed some light in the biological function of this viral protein in the zebrafish. We describe here transcripts induced after injection of adult zebrafish with NV recombinant protein of VHSV. Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in zebrafish internal organs (pooled spleen and head kidney).

2017-06-15 | GSE54076 | GEO

Gene expression profiles after infection of rainbow trout with viral hemorrhagic septicemia virus (VHSV)

2015-03-05 | GSE43285 | GEO

Gene expression profiles after infection of rainbow trout with viral hemorrhagic septicemia virus (VHSV)

Project description:We describe here transcripts induced after intraperitoneal injection of rainbow trout with 2 different viruses, both belonging to strain 23.75 of viral hemorrhagic septicemia virus (VHSV): a deleted Nv gene (dNV) virus and a wild type (wt) virus. Two days after infection, differentially expressed transcript levels from selected immune-related trout genes were studied in internal organs (spleen and head kidney). Fishes were divided in two groups (3 fishes per group). The first group was intraperitoneally injected with 100000 pfu per trout of dNV VHSV, while the second group was injected with 100000 pfu/trout of wt VHSV. All fishes were sacrificed two days post infection.

2015-03-05 | E-GEOD-43285 | biostudies-arrayexpress

Danio rerio

Project description:Gene expression profiles after injection of zebrafish with NV (non virion) recombinant protein from Viral Hemorrhagic Septicemia Virus (VHSV)

| PRJNA234535 | ENA

Zebrafish early transcriptional response to viral haemorrhagic septicemia rhabdovirus (VHSV) challenge vs2

Project description:We describe here the transcripts induced after infection of zebrafish with viral haemorrhagic septicemia rhabdovirus (VHSV). Two days after infection, differentially expressed transcript levels from selected immune-related zebrafish genes were studied in both fins and internal organs (pooled spleen, head kidney and liver).

2015-04-17 | GSE58823 | GEO

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