Transcriptomics

Dataset Information

0

Genome-wide DNA Microarray Unravels Rapid Changes in the Transcriptome of MK-801 treated Rat Brain


ABSTRACT: Background: N-Methyl-D-aspartate receptors (NMDAr), widely located around the central nervous system, are known to be involved in behavioral disorders. Dizocilpine (commonly referred to as MK-801) is a well known non-competitive NMDAr antagonist. Methods: We treated rats with intraperitoneal injection [0.08 (low-dose) and 0.16 (high-dose) mg/kg] of MK-801. In one experiment, 40 min after NaCl (vehicle control) and MK-801 (0.08 mg/kg) injection, electrocorticogram (ECoG) signals were analyzed. In the second experiment, 40 min post-injection, the whole brain of each animal was rapidly removed and separated into amyglada, cerebral cortex, hippocampus, hypothalamus, midbrain and ventral striatum) on ice, followed by analysis using a 4x44K DNA microarray chip. Results: Spectral analysis revealed that a single subcutaneous injection of MK-801 significantly and selectively augmented the power of spontaneous gamma and higher-frequency oscillations. The results from DNA microarray analysis of 4400 genes showed the largest number (up- and down- regulations) of gene expressions in the cerebral cortex (378), midbrain (376), hippocampus (375), ventral striatum (353), amygdala (301), and hypothalamus (201) under low-dose of MK-801. Under high-dose, ventral striatum (811) showed the largest number of gene expression changes. These genes represented... Conclusions: Our results reveal that MK-801 triggered i) an increase in the power of gamma oscillations, and ii) simultaneously caused very early changes in gene expressions in the rat brain, representing a first such inventory of gene expression profiles in brain after acute MK-801 treatment.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE37413 | GEO | 2012/04/25

SECONDARY ACCESSION(S): PRJNA161851

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-11-26 | E-GEOD-63639 | biostudies-arrayexpress
2014-11-26 | GSE63639 | GEO
2012-04-24 | E-GEOD-37413 | biostudies-arrayexpress
2019-03-12 | GSE111708 | GEO
2024-02-06 | PXD046944 | Pride
2003-02-27 | GSE318 | GEO
2003-02-27 | GSE317 | GEO
2003-02-27 | GSE316 | GEO
2003-02-27 | GSE315 | GEO
2020-10-01 | GSE129466 | GEO