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Cardiac gene expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles (set 2)

ABSTRACT: C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE37887 | GEO | 2013/05/02

SECONDARY ACCESSION(S): PRJNA164743

REPOSITORIES: GEO

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Cardiac miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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Cardiac miRNA expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

Project description:C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice. C57BL/6 mice were exposed to 0.000 or 0.162 mg Printex 90 and were sacrificed 1, 3 and 28 days after exposure. Individual total RNA enriched with small RNA from 6 mice per treatment group (control and 0.162mg) was hybridized to 8 x 15K Agilent miRNA slides.

2013-05-02 | E-GEOD-37890 | biostudies-arrayexpress

Cardiac gene expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles (set 1)

Project description:C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice. Individual total RNA (200 ng) from 6 mice per treatment group (control and 0.162mg) and Universal Mouse Reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double-stranded cDNA.

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Cardiac gene expression (random section containing both ventricles) in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles (set 2)

Project description:C57BL/6 mice were exposed to vehicle or 0.162 mg carbon black nanoparticles by intratracheal installation and were sacrificed 1, 3 and 28 days post-exposure. Gene expression was investigated in two sets of mice treated according to the identical exposure protocol. MicroRNAs were investigated in the second set of mice. Individual total RNA (200 ng) from 6 mice per treatment group (control and 0.162mg) and Universal Mouse Reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double-stranded cDNA.

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Hepatic gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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Project description:We investigated pulmonary mRNA profiles of female mice exposed to 0.018, 0.054 and 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to these doses 1, 3 and 28 days after exposure, alongside respective controls. This study demonstrates widespread changes in inflammatory and acute phase response genes that persisted to 28 days at the highest exposure dose. Changes relevant to sterol and terpenoid biosynthetic pathways were also observed.

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Hepatic gene expression in C57BL/6 mice intratracheally instilled with Printex 90 carbon black nanoparticles

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