Project description:3T3 Fibrobalasts grown in the presence of fibronectin to assess MAP kinase signalling pathways. Experiment Overall Design: this experiment include 2 samples and 2 replicates
Project description:Insulin is a potent regulator of protein metabolism. Here we describe a time-resolved map of insulin-regulated protein turnover in 3T3-L1 adipocytes using metabolic pulse-chase labelling and high-resolution mass spectrometry.
Project description:3T3-L1 adipose cells were grown, differentiated and insulin resistance was stimulated by addition of TNF or treatment inlcuding chronic insulin and dexamethasone.
Project description:3T3-L1 fibroblasts are a commonly used in vitro model for adipogenesis. When induced with hormones, they differentiate into mature fat cells. Here, microarrays were used to study 3T3-L1 adipose differentiation through time. Keywords: time course
Project description:To investigate the effect of UBE3D loss on 3T3-L1 differentiation, we generated Ube3d-KO 3T3-L1 cells. We then performed RNA sequencing on undifferentiated WT and KO cells to assess gene expression changes and alterations in RNA 3' end processing.
Project description:Transcriptomics of NIH-3T3 cells treated with typical or atypical RARa modulators to evaluate genetic changes induced by chemical modification of RARa signalling