Transcriptomics

Dataset Information

0

Reduced Retinal Microvascular Density, Improvement in Forepaw Reach, Comparative Microarray and Gene Set Enrichment Analysis using DNA Enzyme Targeting c-jun mRNA


ABSTRACT: Retinal neovascularization is a critical component in the pathogenesis of common ocular disorders that cause blindness, and treatment options are limited. We evaluated the therapeutic effect of a DNA enzyme targeting c-jun mRNA in mice with pre-existing retinal neovascularization. A single injection of Dz13 in a lipid formulation containing N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine inhibited c-Jun expression and reduced retinal microvascular density. The DNAzyme inhibited retinal microvascular density as effectively as VEGF-A antibodies. Comparative microarray and gene expression analysis determined that Dz13 suppressed not only c-jun but a range of growth factors and matrix-degrading enzymes. Dz13 in this formulation inhibited microvascular endothelial cell proliferation, migration and tubule formation in vitro. Moreover, animals treated with Dz13 sensed the top of the cage in a modified forepaw reach model, unlike mice given a DNAzyme with scrambled RNA-binding arms that did not affect c-Jun expression. These findings demonstrate reduction of microvascular density and improvement in forepaw reach in mice administered catalytic DNA.

ORGANISM(S): Mus musculus

PROVIDER: GSE37898 | GEO | 2012/05/11

SECONDARY ACCESSION(S): PRJNA165679

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-05-10 | E-GEOD-37898 | biostudies-arrayexpress
| PRJNA165679 | ENA
2021-12-24 | GSE192340 | GEO
| 2382594 | ecrin-mdr-crc
2020-05-21 | GSE148292 | GEO
2021-10-01 | GSE139947 | GEO
2020-12-22 | GSE160099 | GEO
| 2668668 | ecrin-mdr-crc
2022-07-01 | GSE207271 | GEO
2024-08-20 | GSE241239 | GEO