Genomics

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MicroRNA profiling of human pancreatic alpha and beta cells


ABSTRACT: MicroRNAs (miRNAs) are non-coding RNAs that play a fundamental role in regulation of gene expression affecting differentiation and development. In particular, miRNAs have been described to regulate genes important for pancreatic development and islet function. The aim of this work was to determine the miRNA expression signature in human pancreatic alpha and beta cells. miRNA stability to fixation allowed the study of microRNA in pure populations of human alpha and beta cells sorted by FACS after intracellular staining with glucagon and insulin, respectively. The determination of the specific group of miRNAs expressed in the human pancreatic alpha and beta cells may further the understanding of gene expression regulation of the islet differentiation process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38360 | GEO | 2013/01/30

SECONDARY ACCESSION(S): PRJNA167944

REPOSITORIES: GEO

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